Jan 29, 1999 · Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of the SCN in situ. Immortalized SCN cells generated robust rhythms in uptake of the metabolic marker 2-deoxyglucose and in their content of neurotrophins.

May 4, 2004 · In the rat SCN, ventrolateral (including VIP) neurons receive direct retinal input, and they in turn project to ipsilateral, dorsomedial neurons . Somehow the 22 hr LD cycle has disrupted this cohesive tissue organization and differentially …

May 11, 2005 · To determine whether immortalized cells derived from the rat SCN (SCN2.2) retain these intrinsic rhythm-generating properties, oscillatory behavior of the SCN2.2 transcriptome was analyzed and compared with that found in the rat SCN in vivo using rat U34A Affymetrix GeneChips.

Using a luciferin-luciferase chemiluminescent assay, we have demonstrated that ATP levels fluctuate rhythmically within both SCN2.2 cell cultures and the rat SCN in vivo. SCN2.2 cells generated circadian oscillations in both the production and extracellular accumulation of ATP.

Intracerebroventricular administration of neuromedin S in rats activates SCN neurons and has the capability to induce non-photic type of phase shifts in the circadian rhythm of locomotor activity. It is also possible that NMS along with VIP may have a role in maintenance of circadian rhythmicity.

The suprachiasmatic nucleus (SCN) of the anterior hypothalamus is the principal circadian pacemaker in mammals (Moore and Eichler, 1972; Stephan and Zucker, 1972). In most species, including rat, the SCN has two subdivisions that differ in …

Mar 26, 2004 · Immortalized SCN2.2 cells retain most biochemical and biophysical characteristics of the native rat SCN including the expression of clock genes and circadian regulatory proteins, and its distinctive pacemaker function.

Jul 1, 2016 · The present study aimed to test whether light responsive neurons in the rat SCN display an electrophysiological phenotype that distinguishes them from non‐responsive cells, and also whether this phenotype is affected by circadian rhythms.

Sep 22, 2020 · Forty-two rats were sacrificed to detect circadian rhythmicity in the Gria2 mRNA level using qPCR in the SCN as well as for R/G site editing and alternative flip/flop splicing in the SCN and other parts of the brain.

Valuable tool for studies relating to the regulation of the central mammalian pacemaker function and the development of the suprachiasmatic nucleus (SCN) cell types (Kerafast=EUT500). Doubling time: 28 hours (PubMed= 10213449). Transformant: Psi2 defective retrovirus adenovirus E1A. Omics: Transcriptomics; Microarray.