Rad50 ATPase domains dimerize in trans in the presence of ATP (yellow ovals), forming two functional sites as shown. ATP is engaged by Walker A motif K42 from one Rad50 and signature motif S1202 from the other Rad50.

Mar 26, 2025 · The RAD50 protein belongs to a dynamic complex with Mre11 and Nbs1 (nibrin) in order to facilitate DNA repair function near the site of damage (Qiu and Jun 2021; Bian et al., 2019). The hRAD50 inherited sequence generates a 1,312-amino acid inside the human body (a 153-kDa RAD50 protein).

Here we introduce the DNA repair functions of Rad50-containing complexes and compare their architecture with the SMC complexes cohesin and condensin. ATP binding and hydrolysis are important for all of these protein complexes.

May 22, 2020 · We identified 103 mostly occurred mutations in the Rad50 protein domains and motifs, which only 42 mutations were classified as most deleterious. These mutations are mainly situated at the specific motifs such as Walker A, Q-loop, Walker B, D-loop and signature motif of the Rad50 protein.

We report here the crystal structure of the Thermotoga maritima Rad50 nucleotide-binding domain (tmRad50 NBD) in complex with the Rad50-interacting helix-loop-helix motif of Mre11 (tmMre11 HLH), Mg 2+, AMPPNP, and a dsDNA 15mer.

Human RAD50 deficiency is an autosomal recessive syndrome that has been reported in patients with microcephaly and short stature. Their clinical phenotype resembled Nijmegen Breakage Syndrome. Cells from these patients showed increased radiosensitity with an impaired response to chromosome breaks. [23][24][25] ^ "Human PubMed Reference:".

Apr 7, 2025 · The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends.

Mar 3, 2007 · We propose a model for Rad50 that incorporates both ATPase and adenylate kinase reactions as critical activities that regulate Rad50 functions. The Mre11/Rad50 (M/R) complex plays an important though still largely undefined role …

Mar 21, 2017 · Rad50 ATPase domains dimerize in trans in the presence of ATP (yellow ovals), forming two functional sites as shown. ATP is engaged by Walker A motif K42 from one Rad50 and signature motif S1202 from the other Rad50.

Mar 30, 2025 · RAD50 (RAD50 Double Strand Break Repair Protein) is a Protein Coding gene. Diseases associated with RAD50 include Nijmegen Breakage Syndrome-Like Disorder and Hereditary Breast Ovarian Cancer Syndrome.