Plasmid - Wikipedia
RNA plasmids are non-infectious extrachromosomal linear RNA replicons, both encapsidated and unencapsidated, which have been found in fungi and various plants, from algae to land plants. …
Small RNAs and their mighty roles in plasmid replication
Sep 12, 2023 · A crucial element in plasmid replication is a small RNA molecule termed RNA I that has a pivotal role in regulating the replication process. In this Journal Club article, I …
General plasmids for producing RNA in vitro transcripts with ...
These plasmids provide the tools for the simple, rapid creation of new RNA-coding plasmids to produce milligram quantities of homogeneous in vitro transcripts for all applications.
RNA Plasmids - ScienceDirect
Jan 1, 1989 · In fungi and higher plants, there exists a wide array of resident, nonhorizontally transmissible RNA molecules that replicate in an RNA-dependent fashion. The replication of …
A Comparison of Plasmid DNA and mRNA as Vaccine Technologies
This review provides a comparison of the theoretical issues and experimental findings for plasmid DNA and mRNA vaccine technologies. While both have been under development since the …
Plasmids—Part 2: Perfecting Plasmid Production for IVT mRNA ...
The method provided baseline separation of isoforms and yielded SC-rich fractions (>90%) virtually free from RNA with levels of genomic DNA (1.3 ± 0.3% µg gDNA/µg SC plasmid DNA) …
Plasmid - an overview | ScienceDirect Topics
RNA plasmids are found as both single-stranded and double-stranded forms and replicate in a manner similar to certain RNA viruses. The RNA plasmid encodes RNA-dependent RNA …
Unraveling Plasmids: A Comprehensive Guide - CD Genomics
To understand how a plasmid works, scientists use a plasmid map. This map shows key parts of the plasmid, like where it starts copying itself and any genes it carries. Below are key steps for …
RNA motif plasmid cloning backbone - Addgene
Digest plasmid with BsmBI and ligate RNA motif of interest. The RNA motif of interest would be in the 3UTR and flanked by BoxB motifs. Oligo cloning can be performed similarly to sgRNA …
In summary, rNA I has a pivotal role in the replication of ColE1-type plasmids by regulating the activity of rNA II. Its mecha-nism of action and the factors that influ-ence its...