PaqCI is an enzyme that requires multiple recognition sites to achieve complete digestion. NEB has therefore included “PaqCI Activator” which can be added to the reaction with 1:1 ratio (i.e. 1 ul enzyme to 1 ul PaqCI Activator).

PaqCI is a Type IIS restriction enzyme that has a 7-base pair recognition sequence, useful for avoiding domestication issues in Golden Gate Assembly. This enzyme requires more than one site to efficiently cleave. To achieve optimal cleavage, PaqCI Activator is …

Apr 7, 2021 · In this blog post, read about how PaqCI (an AarI isoschizomer) can be used for simple to complex 24-fragment assembly, achieving our highest level of efficiency and fidelity yet, and with less concerns regarding the domestication of internal sites due to its 7 base-pair recognition sequence.

May 22, 2023 · Here we describe biochemical analyses of DNA cleavage by the Type IIS PaqCI restriction endonuclease and a series of molecular structures in the presence and absence of multiple bound DNA targets.

T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified [1] as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. [2] Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. [3]

Name: pAQ-B Insert: Wild-type Aequorin cDNA (HindIII-EcoRI from pAQ440: ΔBamHI) Vector: pUC9-2

PaqCI is an enzyme that requires multiple recognition sites to achieve complete digestion. NEB has therefore included “PaqCI Activator” which can be added to the reaction with 1:1 ratio (i.e. 1 ul enzyme to 1 ul PaqCI Activator).

Paq5000 Hotstart DNA polymerase is a robust, economic alternative to Taq DNA polymerases utilizing hot start technologies. It has been optimized to provide improved hot start capability, higher specificity, and better PCR yields with reduced cycling time.

The enzyme is a member of the ammonia lyase family, which cleaves carbon–nitrogen bonds. Like other lyases, PAL requires only one substrate for the forward reaction, but two for the reverse. It is thought to be mechanistically similar to the related enzyme histidine ammonia-lyase (EC:4.3.1.3, HAL). [ 8 ]

One unit is defined as the amount of enzyme required to digest 1 µg of pNEB193 DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Not sensitive to CpG, dcm, or dam methylation. How can the efficieny of PacI be increased? Is PacI inhibited by salt? What is …