PA-GFP is a photoactivatable green fluorescent protein published in 2002, derived from Aequorea victoria.

Sep 15, 2005 · Recently, a novel methodology has emerged with the development of so-called photoactivatable fluorescent proteins (PAFPs), which are capable of pronounced changes in their spectral...

Apr 25, 2017 · Photoactivatable fluorescent proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon exposure to a specific wavelength of light.

This report covers the two-photon activation and excitation properties of the PA-GFP, a photoactivatable variant of the Aequorea victoria green fluorescent protein in the spectral region from 720 to 920 nm.

Here, we describe methods to tag and express proteins with PA-GFP, locally photoactivate the recombinant protein and record the dynamic behavior of the photoactivated molecules in live cells. Use of photoactivatable proteins is a powerful approach to examine dynamic processes, including spindle formation, in diverse cells.

May 4, 2017 · Being photoactivated with the help of violet light, PA-GFP (photoactivatable green fluorescent protein) switches its absorption maximum from 400 to 504 nm (s. Figure 2). Therefore its fluorescence increases ca. 100-fold when excited with a wavelength of 488 nm, what is reflected in a sharp contrast between activated and non-activated proteins.

We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 100 times when excited by 488-nanometer light and remains stable for days under aerobic conditions.

Oct 1, 2009 · Three years later, Patterson et al. generated the first photoactivatable GFP (PA-GFP), which turned out to be a crucial advance for super-resolution fluorescence microscopy approaches. The...

Photoactivatable green fluorescent protein (paGFP) exhibits peculiar photo-physical properties making it an invaluable tool for protein/cell tracking in living cells/organisms. paGFP is normally excited in the violet range (405 nm), with an emission peak centred at 520 nm. Absorption cross-section at 488 nm is low in the not-activated form.

Photoactivatable GFP variant that becomes fluorescent upon irradiation with UV light. Sequence Author: Lippincott-Schwartz Lab. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. BsrGI is typically used at 37°C, but is even more active at 60°C. * Blocked by Dam methylation. * Blocked by Dcm methylation.