Aug 3, 2015 · Qualitative assessment and comparison of p62 staining between healthy and disease sections or disease subtypes will help target further investigation into the potential roles for autophagy in a variety of disorders.

Herein, this protocol describes the predominant method used for the research of autophagy, which mainly focuses on the immunofluorescence staining of cellular LC3, P62, and ULK1 in response to normoxia and hypoxia, by presenting the …

Immunohistochemistry for p62, LC3 and/or LAMP2 was compared with routine histological and histochemical staining, in order to evaluate their role as diagnostic tools for the differentiation of autophagic vacuolar myopathies.

Overall, 62% of the inflammatory myopathy muscle biopsies had some type of positive staining for p62. This was most prevalent in muscle biopsies from those with sIBM (92%), IMNM (87%), and DM (57%). Staining for p62 was also present in PM (29%), neurogenic disorders (31%) and other types of myositis (26%).

May 6, 2022 · We studied LC3 and p62 expression using immunohistochemistry in a large cohort of 466 stage I/II non-small cell lung cancer (NSCLC) using a tissue microarray. We evaluated dot-like cytoplasmic expression of LC3 and dot-like, cytoplasmic and nuclear staining for p62 in relation to clinico-pathological parameters.

Immunohistochemical detection of ubiquitin-binding protein p62 is a sensitive and reproducible method to identify grain pathology in AGD. Interpretation Provides information to assist in interpretation of the test results

Emphasizing the importance of proper antibody and epitope unmasking methods for maximum sensitivity, we recommend p62 immunodetection as a screening stain for diagnostic practice. The demonstration of proteinaceous inclusions in the brain is the key step in the pathological diagnosis of degenerative dementias.

Mar 27, 2018 · Immunofluorescence staining of P62 reveals a clear, punctate pattern localized into cytoplasm. LC3, another autophagy biomarker except for P62, also diffuses evenly in cytoplasm in the absence of autophagy.

May 11, 2021 · Here, we assessed the importance of SQSTM1 in human induced pluripotent stem cell (iPSC)-derived cortical neurons through the knockout of SQSTM1. We show that SQSTM1 depletion causes altered mitochondrial gene expression and functionality, as well as autophagy flux, in iPSC-derived neurons.

Feb 3, 2017 · LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry.