A nuclear localization signal or sequence (NLS) is an amino acid sequence that 'tags' a protein for import into the cell nucleus by nuclear transport. [1] Typically, this signal consists of one or more short sequences of positively charged lysines or arginines exposed on the protein surface. [ 1 ]

Therefore, we propose NLS-tagging as an effective and fast alternative strategy to the common N- or C-terminal tag integration methods for the characterization of protein complexes formed by TFs and the identification of their DNA binding regions.

May 22, 2021 · Nuclear localization signals (NLS) are generally short peptides that act as a signal fragment that mediates the transport of proteins from the cytoplasm into the nucleus.

Specifically, we will highlight the most significant advancements in NLS-tagging for various therapeutic platforms, with a particular focus on structural elements such as NLS tag densities and orientations, conjugation mode, and diseased cellular environments.

A nuclear localization signal or sequence (NLS) is an amino acid sequence which tags a protein for import into the cell nucleus by nuclear transport. Typically, this signal consists of one or more short sequences of positively charged lysines (K) or arginines (R) exposed on the protein surface.

Dec 1, 2014 · We demonstrate the effectiveness of this approach with the transcription factors Fli-1 and Irf2bp2, which cannot be tagged at their extremities without loss of function. This resulted in the identification of novel proteins partners and a new hypothesis on the contribution of Fli-1 to hematopoiesis. © The Author (s) 2014.

Jan 1, 2000 · Proteins enter the nucleus from the cytoplasm through a 25-nm active channel by a facilitated process that often requires the presence of a nuclear localization signal (NLS) on the transported protein as well as soluble transport factors.

We report here six different NLS classes that specifically bind to distinct binding pockets of importin α. By screening of random peptide libraries using an mRNA display, we selected peptides bound by importin α and identified six classes of NLSs, including three novel classes.

Jun 6, 2015 · Among the studied proteins, NLS c-Myc-eGFP exhibited an increase in nuclear intensity by 160% compared to that in the cytosol while NLS SV40-eGFP, one of the most routinely used NLS tags, exhibited an increase in intensity of only ca. 45%, with a substantial amount of protein remaining in the cytosol.

A nuclear localization signal (NLS) is encoded by AA379–386, which also associates with the cellular nuclear importation machinery (Kim et al., 1997; Fischer et al., 1997). Sequences within the Arg-Gly rich regions of LR1 and LR2 may also function as NLSs due to …