Ready-to-use AAV PHP.eB particles produced from CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression.

May 22, 2021 · Classical NLS sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker, for measurement of the nuclear-cytoplasmic shuttling rate in living cells or for single molecules to track how a single protein travels through the nucleus [87,88,89].

Plasmid nls-EGFP from Dr. Rob Parton's lab contains the insert nls-EGFP and is published in Dev Cell. 2015 Nov 23;35(4):513-25. doi: 10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12. This plasmid is available through Addgene.

Jul 31, 2015 · Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells.

Jul 31, 2015 · Our NLS-tagging procedure is ideal for use in artificial sequestration of proteins in the nucleus. Classical nuclear localization signal (NLS) sequences have been used for artificial localization of green fluorescent protein (GFP) in the nucleus as a positioning marker or for measurement of the nuclear-cytoplasmic shuttling rate in living cells.

To test the RNA binding of basic peptides fused to GFP, we performed an in vitro RNA pulldown assay using NLS-R7-GFP immobilized to sepharose beads via the GFP binding protein (GBP) 39 (Fig. 4C, scheme).

Nov 24, 2024 · The ELL2 NLS relocalizes GFP-β-Gal fusion constructs to the nucleus. (A) Overview of an established NLS mapping system (pHM830) encoding an N-terminal green fluorescent protein (GFP) and a C-terminal beta-galactosidase …

We report here six different NLS classes that specifically bind to distinct binding pockets of importin α. By screening of random peptide libraries using an mRNA display, we selected peptides bound by importin α and identified six classes of NLSs, including three novel classes.

Our results provide new experimental evidence to support the report, showing that the NLS-contained GFP-F14SN and GFP-F19SQ fragments can be imported into the nucleus even if phosphorylation were blocked by mutations of serine residues.

Feb 17, 2020 · We removed the nuclear localization signal (NLS) from MCP–GFP for efficient trapping of cytosolic mRNAs.