Munc-18 (an acronym for mammalian uncoordinated-18) proteins are the mammalian homologue of UNC-18 (which was first discovered in the nematode worm C. elegans [1] [2]) and are a member of the Sec1/Munc18-like (SM) protein family.

In this study, we found that Munc-18-1 as well as Syntaxin1A is indispensable for radial migration of cortical neuron during brain development. Munc18–1 was shown to regulate post-Golgi transport of vesicles to the plasma membrane as well as subsequent vesicle fusion at cell surface for proper distribution of proteins crucial for neuron ...

Sep 23, 2019 · Munc18-1 and Munc13-1 orchestrate SNARE complex formation by an NSF-SNAP-resistant mechanism 11 whereby Munc18-1 first binds to a self-inhibited “closed” conformation of syntaxin-1 12,13 (Fig...

Jan 8, 2019 · Acceptor liposomes (Munc18-1/Syx1) contain 55% POPC, 20% POPE, 18% DOPS, 2% PIP2, and 5% DAG.

Jul 14, 2021 · Affiliations 1 Section Functional genomics, Department of Clinical Genetics, Center for Neurogenomics and Cognitive Research, Universitair Medisch Centrum, Amsterdam1081 HV, The Netherlands.; 2 Department of Functional Genomics, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, Amsterdam1081 HV, The Netherlands.; 3 Department of Functional Genomics, Center for ...

Mammalian uncoordinated-18 (Munc 18) protein belongs to family of the Sec1/Munc18-like (SM) proteins. The Munc-18 protein is considered as essential component of trans-SNARE complex crucial for the exocytosis of secretory granules ( Zilly et al., 2006 ).

Munc-18-1 is a 67-kDa neuronal protein that binds tightly to syntaxin 1 and functions in synaptic vesicle exocytosis (Hata, Y., Slaughter, C. A., and Südhof, T. C. (1993a) Nature 366, 347-351). We have now characterized a new Munc-18 isoform, Munc-18-2, that exhibits 63% amino acid sequence identity with Munc-18-1.

Sep 28, 2018 · Worms expressing mutant unc-18 variants paralyzed significantly faster compared to either N2 C. elegans or unc-18 null worms rescued with WT unc-18 (Fig. 1e), and exhibited reduced...

Munc-18 is thought to serve multiple roles in exocytosis, the first being as an inhibitory protein which prevents assembly of the SNARE complex at inopportune times and which plays an important role in mediating vesicle docking (de Wit, 2010; Dulubova et al., 1999; Toonen et al., 2006; Voets et al., 2001; Wojcik and Brose, 2007; Yang et al., 2000).

In this study, we have found that mutations identified in C. elegans [unc-18(b403)] and D. melanogaster (rop G11 and rop A19) also reduce the affinity for syntaxin1A when introduced into homologous residues in mammalian Munc18-1.