mKeima :: Fluorescent Protein Database
Dec 14, 2018 · mKeima is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 2006, derived from Montipora sp. 20. It has high acid sensitivity.
A Sensitive and Quantitative mKeima Assay for Mitophagy via FACS
Dec 26, 2019 · The mKeima assay can be used for both confocal imaging and FACS analysis to provide a thorough picture of mitophagy with a wide dynamic range. The Keima fluorophore has bimodal excitation under neutral and acidic pH conditions.
A fluorescence-based imaging method to measure - Nature
Jul 13, 2017 · We created a transgenic mouse model that expresses the pH-dependent fluorescent protein mt-Keima in order to more readily assess mitophagy. Keima is a pH-sensitive, dual-excitation ratiometric...
The TMEM192-mKeima probe specifically assays lysophagy and …
Oct 6, 2023 · In this study, we developed a lysophagy flux assay using the TMEM192-mKeima probe, which allowed us to assess lysophagic activity separately from other pathways involved in the lysosomal damage response.
Measuring In Vivo Mitophagy: Molecular Cell - Cell Press
Nov 19, 2015 · Keima is a coral-derived protein that exhibits both pH-dependent excitation and resistance to lysosomal proteases. Comparison of a wide range of primary cells and tissues generated from the mt-Keima mouse revealed significant variations in basal mitophagy.
Quantification of mitophagy using mKeima-mito in cultured …
Apr 1, 2022 · The use of an AAV produces efficient expression of mKeima-mito in difficult to transfect cells, including primary cell culture models. mKeima-mito grants accurate quantification of mitophagy in time-lapse images of live primary RPE cultures.
Methods for Monitoring Mitophagy Using mt-Keima
Aug 9, 2024 · Mitochondria-targeted Keima (mt-Keima) is a pH-sensitive, acid-stable fluorescent protein used for the quantification of mitophagy. Mt-Keima contains a mitochondrial matrix targeting sequence and has bimodal excitation with peaks at 440 nM in neutral environments and 586 nM in acidic environments.
Monitoring the autophagy-endolysosomal system using …
Jun 8, 2020 · In this study, by exploiting merits of mKeima and LC3 combined, we newly developed a single fluorescent protein-based and pH-sensitive probe, mKeima-LC3B, as a novel analytical tool for the autophagy-endolysosomal system. We generated transgenic (tg) mice that were expressing mKeima-LC3B.
Excited State Proton Transfer in the Red Fluorescent Protein mKeima
Sep 9, 2009 · mKeima is the only red fluorescent protein shown to employ ESPT for emission, utilizing a novel proton relay different from that found in GFP and provides a new framework for study.
mKeima Dye Profile - FluoroFinder
<html>mKeima is a monomric, pH-sensitive red fluorescent protein with excitation and emission maximums of 440 nm and 620 nm within the range of pH6-8, respectively. Below pH 6, the excitation max shifts to 486 nm with the same emission peak at 620 nm. mKeima was first derived from the stony coral, <i>Montipora sp 20.</i></html>