Jun 14, 2017 · In this study, we used machine learning algorithms to develop a simple and fast imaging-based approach that enables automated identification of different macrophage functional phenotypes using...

Here we report that interferon (IFN) γ priming of naive macrophages induces store-mediated Ca 2+ entry and inhibition of Ca 2+ entry impairs polarization to M1 inflammatory phenotype.

We developed anti-BTN2A1 monoclonal antibodies (mAbs), and notably, one clone demonstrated a robust inhibitory effect on M2-like macrophage differentiation, inducing a shift toward an M1-like phenotype both in vitro and ex vivo in TAMs from patients with cancer.

Jul 2, 2024 · There are two predominant macrophage profiles: inflammatory or classically activated M1, characterized by TLRs activation, production of IL-1, IL-6, TNFα, IL-12, and high amounts of nitric...

1 day ago · Activated macrophages can be divided into two distinct subgroups: pro-inflammatory (killing) M1 and anti-inflammatory (repairing) M2 macrophages. M1 macrophages show increased antigen presentation ...

Mar 21, 2025 · The numbers of mice are indicated. The results are depicted as the means ± SEM, *P < 0.05, **P < 0.01, and ***P < 0.001. ns, not significant. In vivo liver metastasis model of colon carcinoma also showed that Parkin deficiency extended mouse survival (fig. S1H). ... Dot plot of selected genes from M1 macrophage signatures. (D) Dot plot of ...

2 days ago · However, the transfection efficiency between M1- and M2-type macrophages in the injured spinal cord had no significant difference (fig. S8D). These results suggested that PS/LNPs could effectively enhance the mRNA delivery to the injured spinal cord, especially preferentially targeted macrophages. ... All data are presented as means ± SEM and ...

Feb 25, 2025 · Bioinformatics and in vitro cell experiments revealed that DSCM@EVs promote the reprogramming of M1 macrophages to the M2 phenotype, reducing inflammatory responses and facilitating NSC differentiation. ... (SEM). Samples were pre-fixed in 2.5% glutaraldehyde for 6 h, washed thrice with deionized water for 30 min each, dehydrated in a series of ...

Macrophages play vital roles in maintaining immune homeostasis in the large and distal airways. Key macrophage functions include phagocytosis of microbial pathogens, release of cell signaling molecules, and participation in tissue remodeling [1 – 3].

Mar 20, 2025 · The values on the graphs are mean ± SEM values of supernatants from 3–4 independently performed experiments. *p < 0.01 and ***p < 0.0005 compared to PMA. 20 µL of total ... The hyperactivation of some of these factors was associated with macrophage M1 polarization during bacterial or viral infection . Tax protein participates in ...