Here we report synthesis of four m 3 G-CAP constructs and the effective attachment of these onto oligonucleotides (ONs) using Cu (I) [3 + 2] cycloaddition (“click chemistry”).

Jul 6, 2005 · Here we report the crystal structure of the m 3 G-cap-binding domain of snurportin1 with bound m 3 GpppG at 2.4 Å resolution, revealing a structural similarity to the mRNA-guanyly-transferase. Snurportin1 binds both the hypermethylated cap and the first nucleotide of the RNA in a stacked conformation.

Aug 17, 1990 · Here the involvement by the 5′-terminal cap of snRNA molecules in the nucleo-cytoplasmic transport of UsnRNPs has been studied by microinjection of synthetic U1 RNA molecules into frog oocytes; the U1 RNA bore either the normal cap (m 3 …

The RNA components of small nuclear ribonucleoproteins (U snRNPs) possess a characteristic 5'-terminal trimethylguanosine cap structure (m3G cap). This cap is an important component of the nuclear localization signal of U snRNPs. It arises by hypermethylation of a cotranscriptionally added m7G cap.

Competition studies with snRNPs showed that the Sm core domain of U1 snRNPs contains a nuclear location signal that acts independently of the m3G cap.

We tested whether m3G had a signaling role with a chemically synthesized m3GpppG cap (19). We attached this cap at the 5' end of U1 RNA by using it as primer for RNA polymerase SP6-directed synthesis of Ul snRNA from a suitably constructed plasmid. In this way, capped Ul snRNA and related derivatives were prepared in. vitro.

Aug 17, 1990 · Here the involvement by the 5'-terminal cap of snRNA molecules in the nucleo-cytoplasmic transport of UsnRNPs has been studied by microinjection of synthetic U1 RNA molecules into frog oocytes; the U1 RNA bore either the normal cap (m3G) or …

The trimethylguanosine (m3G) cap present at the 5′ end of small nuclear RNAs (snRNAs) has been proposed as an effective nuclear localization signal (NLS) for nucleus-targeting therapeutics such as antisense oligonucleotides.

The RNA components of small nuclear ribonucleoproteins (U snRNPs) possess a characteristic 5′-terminal 2,2,7-trimethyl-guanosine CAP structure (m3G-CAP). This cap is an important component of...

Feb 10, 2009 · We report on the synthesis and possible applications of a natural RNA 5′-end nuclear localization signal composed of a 2,2,7-trimethylguanosine cap (m 3 G-CAP). The cap is found in the small nuclear RNAs that are constitutive part of the small nuclear ribonucleoprotein complexes involved in nuclear splicing.