Oct 13, 2021 · In this study, we have immunized an alpaca with lupine protein extracts and retrieved nanobodies (Nbs). Nevertheless, the target antigen has been recognized as Lup an 1, which has been classified as β-conglutin, and confirmed to connect with lupine allergy.

Nadal et al. have recently selected an aptamer against the food allergen Lup an1 (from lupine) . In the same context, Tran et al. have successfully designed specific aptamers strands against food allergens including lysozyme and Ara h 1 .

Oct 18, 2019 · The current study developed a new highly specific, sensitive and accurate ELISA method to detect, identify and quantify the lupin main allergen β-conglutin (Lup an 1) protein in natural and processed food.

Apr 17, 2012 · Using in vitro selection, high affinity DNA aptamers to the food allergen Lup an 1, ß-conglutin, were selected from a pool of DNA, 93 bases in length, containing a randomised sequence of 49 bases. ß-conglutin was purified from lupin flour and chemically crosslinked to carboxylated magnetic beads.

Oct 13, 2021 · In this study, we have immunized an alpaca with lupine protein extracts and retrieved nanobodies (Nbs). Nevertheless, the target antigen has been recognized as Lup an 1, which has been classified as ?-conglutin, and confirmed to connect with lupine allergy.

Apr 15, 2014 · A sensitive method for the rapid and sensitive detection of the anaphylactic food allergen Lup an 1 (β-conglutin) exploiting fluorescence resonance energy transfer (FRET) has been developed. A high affinity dimeric form of a truncated 11-mer aptamer against β-conglutin was used, with each monomeric aptamer being flanked by donor/acceptor ...

Using in vitro selection, high affinity DNA aptamers to the food allergen Lup an 1, ß-conglutin, were selected from a pool of DNA, 93 bases in length, containing a randomised sequence of 49 bases. ß-conglutin was purified from lupin flour and chemically crosslinked to carboxylated magnetic beads.

The major lupin allergen Lup an1 (β-conglutin) has sequence similarities to the major peanut allergen Ara h1. Generally, lupin allergens are resistant to thermal, chemical, and proteolytic degradation.

The major lupin allergen Lup an1 (β-conglutin) has sequence similarities to the major peanut allergen Ara h1. Generally, lupin allergens are resistant to thermal, chemical, and proteolytic degradation.

Jan 1, 2012 · Using in vitro selection, high affinity DNA aptamers to the food allergen Lup an 1, ß-conglutin, were selected from a pool of DNA, 93 bases in length, containing a randomised sequence of 49...