Ku70 is a heterodimeric protein made up of Ku70 and Ku80, which together form Ku. In humans, is encoded by the XRCC6 gene. [5] [6] Ku70 plays a critical role in the DNA repair, maintenance and many other cellular processes.

The heterodimer Ku70/80 (Ku) is a central actor of the c-NHEJ as it rapidly recognizes broken DNA ends in the cell and protects them from nuclease activity. It subsequently recruits many c-NHEJ effectors, including nucleases, polymerases, and the DNA ligase 4 complex.

Jan 26, 2024 · Microbial DNA and mislocalized DNA from damaged host cells can activate different host responses that shape disease outcomes. Here, we show that mice and humans lacking a single allele of the DNA repair protein Ku70 had increased susceptibility to the development of intestinal cancer.

Ku70 is a DNA repair enzyme found in the cytosol and nucleus but not associated with mitochondria. Ku70 was identified as a Bax binding protein using yeast to screen mammalian libraries for proteins that would inhibit the toxicity of Bax overexpressed in yeast [66]. Full-length Ku70 or the C-terminal 113 amino acids of Ku70 suppressed apoptosis ...

The knockdown studies and the reporter assay revealed that Ku70 is a novel DNA sensor inducing the IFN-lambda1 activation. The functional analysis of IFNL1 promoter revealed that PRDI and ISRE sites are predominantly involved in the DNA-mediated IFNL1 activation.

Jan 26, 2024 · An immune system protein named Ku70 may be triggered to combat bowel cancer after being "switched on" by a cocktail of drugs, according to a new paper in the journal Science Advances.

May 10, 2022 · The KU70-K570R (mouse Ku70-K568R) mutation dampens the anti-apoptosis activity of KU70. Our study identifies KU70 as a non-histone substrate of SETD4, discovers a post-translational modification of KU70, and uncovers a role for SETD4 and KU70-K570 methylation in the suppression of apoptosis.

The current study demonstrated that the translocation of Ku70 from the nucleus to the cytoplasm is an early and required event in Ku70/DNA‐mediated IFN‐λ1 induction and acetylation plays an essential role in modulating ku70’s cytosolic DNA sensing …

Oct 21, 2021 · We explored the unique structure of Ku70 binding with DNA; illustrated, with evidence, how Ku70, as a nuclear protein, responds to extracellular DNA stimulation; and summarized the mechanisms of the Ku70-involved innate immune response pathway.

May 1, 2004 · Ku is a heterodimer composed of two subunits — Ku70 and Ku80 — that binds to DNA ends in a sequence-independent manner. From crystallographic studies, we know that it is shaped like an asymmetric...