In T cells, the Tec family kinase, ITK, phosphorylates PLCγ1 at tyrosine 783 (Y783) leading to activation of phospholipase function and subsequent production of the second messengers IP 3 and DAG. In this work we demonstrate that PLCγ1 can be primed for ITK mediated phosphorylation on Y783 by a specific region of the adaptor protein, SLP-76.

Itk functions in TCR signaling via its interactions in the LAT-SLP-76 signaling complex, where it participates in activation of PLC-γ and the downstream generation of DAG and IP 3, as well as regulation of the actin cytoskeleton via interactions with Vav.

The nonreceptor tyrosine kinase Itk plays a key role in TCR-initiated signaling that directly and significantly affects the regulation of PLCγ1 and the consequent mobilization of Ca2+. Itk also participates in the regulation of cytoskeletal ...

Aug 27, 2021 · Interleukin-2–inducible T cell kinase (ITK) is a critical component for the optimal activation of phospholipase-C-gamma-1 (PLC-γ1), the enzyme that cleaves the membrane-embedded phosphatidylinositol bisphosphate into equimolar amounts of two second signaling messengers: inositol triphosphate (IP 3) and diacylglycerol (DAG).

Jan 22, 2022 · Here, we review the current molecular level understanding of how the TEC kinases (ITK and BTK) and PLCγ1/2 are autoinhibited prior to activation of cell surface receptors, how TEC kinases are activated to specifically recognize the PLCγ substrate, and how conformational changes induced by phosphorylation trigger PLCγ activation.

Apr 1, 2005 · Now, several studies indicate that the TEC-family kinase ITK (interleukin-2-inducible T-cell kinase) is also required for the actin reorganization and cell polarization that occurs...

Apr 17, 2007 · ITK (IL-2-inducible T cell kinase), a Tec family protein tyrosine kinase (PTK), is one of three PTKs required for T cell antigen receptor (TCR)-induced activation of phospholipase C-γ1 (PLC-γ1).

In functional experiments that mimic Tec up-regulation, we find that Tec overexpression in lymphocyte cell lines is sufficient to induce phospholipase Cγ (PLC-γ) phosphorylation and NFAT (nuclear factor of activated T cells) activation. In contrast, overexpression of Btk, Itk, or Bmx does not induce NFAT activation.

In this study, we have produced soluble full-length wild-type ITK protein by co-expressing an N-terminal solubility-tagged ITK construct with a kinase-specific co-chaperone CDC37 in an insect cell line.

Aug 8, 2017 · Activation of the T cell receptor leads to the formation of a signalosome complex between SLP-76, LAT, PLC-γ1, Itk, and Vav1. Recent studies have revealed the existence of both positive and negative feedback pathways from SLP-76 to the apical kinase in the pathway, Lck.