May 26, 2017 · Indirect immunofluorescence, or secondary immunofluorescence, is a technique used in laboratories to detect circulating autoantibodies in patient serum. It is used to diagnose autoimmune blistering diseases.

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Liver-related autoantibodies are crucial for the correct diagnosis and classification of autoimmune liver diseases (AiLD), namely autoimmune hepatitis types 1 and 2 (AIH-1 and 2), primary biliary cirrhosis (PBC), and the sclerosing cholangitis variants in adults and children.

Indirect immunofluorescence (IIF) is a two-step serological technique in which a labeled antibody is used to detect a specific antibody against an antigen in the serum. For this reason, suspected serum is added to a glass slide with antigen-coated fields, followed by …

Indirect immunofluorescence (IIF) plays an important role in immunological and immunometric assays for detecting and measuring autoantibodies. This technology was the first multiplex method used to detect cardinal autoantibodies for the diagnosis of autoimmune diseases.

Feb 15, 1983 · The indirect immunofluorescence (IFL) test is the method of choice for the detection of new organ-specific autoantibodies. It is also the standard method of detection of antibodies to pancreatic islet cells, adrenal cortex, and …

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Twenty-seven patients affected by ulcerative colitis or Crohn's disease were submitted to a secretin-cerulein test, oral glucose test (OGT) and to indirect immunofluorescence (IFL) for detection of autoantibodies against exocrine and endocrine tissue.

Apr 2, 2025 · Scientists have developed a blood test for Alzheimer’s disease that can help diagnose the neurodegenerative disease while also indicating how far it has progressed. The results could help doctors...