Isoelectric focusing (IEF), also known as electrofocusing, is a technique for separating different charged molecules by differences in their isoelectric point (pI). [1][2] It is a type of zone electrophoresis usually performed on proteins in a gel that takes advantage of the fact that overall charge on the molecule of interest, i.e. the net char...

Novex IEF Gels effectively create a pH gradient so proteins separate according to their unique pI. These gels can be used to determine the pI or to detect minor changes in a protein due to deamination, phosphorylation, or glycosylation.

IEF gels are cast with carrier ampholytes to create a pH gradient within the gel. Proteins migrate to their pI, the pH at which their net charge is zero. IEF gels contain no denaturing agents, allowing one-dimensional separation under native conditions.

Novex™ pH 3-10 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI).

Precast immobilized pH-gradient (IPG) gels and gel powders for separating/analyzing proteins or other biomolecules by isoelectric focusing (IEF); for molecular size and pH formulations, well and length configurations.

The first dimension in a 2-D gel electrophoresis experiment involves the separation of proteins according to their isoelectric point (pI) by isoelectric focusing (IEF). IEF works by applying an electric field to protein within a pH gradient.

Browse our selection of isoelectric focusing gels and strips for separating and analyzing peptides and proteins. These essential electrophoresis tools include IPG strips and precast gels designed for isoelectric focusing protocols. Our range features Novex pH 3-10 and pH 3-7 IEF Protein Gels, as well as ZOOM IPG Strips in various pH ranges.

Isoelectric focusing (IEF) is mostly run in horizontal gels because of several reasons: - As IEF separates only according to the charge, the gel matrix must contain large pore sizes. Such a soft gel can slide down between vertical glass plates. The …

Instructions are provided below for electrophoresis of Novex® IEF Gels using the XCell SureLock ® Mini-Cell. For details, refer to the Novex ® Technical Guide available at

Choose this midi pH 3–10 Criterion IEF Gel to separate proteins based on their isoelectric point (pI). The nondenaturing gel contains 2% ampholytes, which form a linear pH gradient on application of current.