Lipid nanoparticle mRNA systems containing high levels of …
Incubation (24 h) of HuH7 cells with LNP Luc mRNA formulations (0.3 μg mRNA/mL) containing 40 mol % ESM resulted in a 100-fold increase in luciferase expression compared with the 10 …
Multiomics analysis of naturally efficacious lipid nanoparticle …
Jul 6, 2023 · We find that the most efficacious LNP-corona complexes were enriched with high-density lipoprotein (HDL) and, compared to the commonly used corona-biomarker …
the most potent LNP formulation tested in A549 cells (Figure 3) and induced slightly greater luciferase activity at the highest concentration tested in Huh7 cells. All LNP formulations tested …
A high-throughput Galectin-9 imaging assay for quantifying ... - Nature
Feb 16, 2021 · We have developed a high-throughput imaging-based endosomal escape assay utilising a Galectin-9 reporter and fluorescently labelled mRNA to probe correlations between …
“LNP之父”Peter Cullis再改良LNP配方，40%辅助脂质配比改善LNP …
Jun 16, 2023 · LNP作为已获上市产品验证的有效、安全递送系统，已被广泛开发用于治疗诸如癌症、动脉粥样硬化、心力衰竭和罕见病等疾病的核酸类药物。 上市产品中使用的LNP通常由 …
Kinetics of RNA-LNP delivery and protein expression
Apr 1, 2024 · We describe LNP delivery as a sequence of stochastic transfer processes and review a mathematical model of subsequent protein translation from mRNA. We compile …
LNP uptake and eGFP expression in Huh7 cells exposed to LNPs …
Lipid nanoparticles (LNPs) have emerged as potent carriers for mRNA delivery, but several challenges remain before this approach can offer broad clinical translation of mRNA therapeutics.
Efficient hepatic delivery and protein expression enabled by optimized ...
Dec 1, 2020 · Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs. mRNAs …
Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 …
Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is …
Lipid nanoparticle-mediated lymph node–targeting delivery of …
In this study, we explored an endogenously LN-targeting lipid nanoparticle (LNP) without the modification of any active targeting ligands for developing an mRNA cancer vaccine.