The human leukocyte antigen (HLA) system is a complex of genes on chromosome 6 in humans that encode cell-surface proteins responsible for regulation of the immune system. [1] . The HLA system is also known as the human version of the major histocompatibility complex (MHC) found in many animals. [2]

Apr 24, 2023 · Conventionally, HLA classes are detected by the polymerase chain reaction (PCR). PCR uses variable exon sequences that encode the first amino-terminal from the HLA domains. From there, the HLA sequences from the database are used in hybridization with the amplified PCR products.

The human leukocyte antigen (HLA) system is one of the most crucial host factors influencing disease progression in bacterial and viral infections. This review provides the basic concepts of the structure and function of HLA molecules in humans. ...

HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample.

May 23, 2021 · 1. PCR with sequence specific primers (PCR-SSP) One commonly applied approach is to use panels of sequence specific DNA primers which amplify particular HLA alleles or allele groups. PCR-SSP is a very rapid test that can be performed in 3 – 4 hours and is commonly used for typing deceased organ donors when speed is an important ...

We describe a new set of HLA-A, -B, and -C locus-specific primers for the polymerase chain reaction (PCR) amplification of the whole coding sequence of these genes from complementary DNA (cDNA). We describe their use for typing and for the production of a library of recombinant HLA class I genes.

Oct 16, 2024 · NGS library preparation for HLA typing uses long-range PCR amplicons that span the entire gene sequence, allowing for high-resolution typing. Long-range PCR, however, increases the...

The polymerase chain reaction (PCR)-based technology is used for clinical HLA typing. 21 The first method developed uses sequence-specific oligonucleotide probe (SSOP).

Consistent design of PCR primers along with use of the most up-to-date sequence alignments, are key features of successful and accurate PCR-SSP HLA typing.

Nov 7, 2011 · The PCR-SSOP technique (Cao et al., 1999) is more amenable to high-throughput HLA typing than PCR-SSP. HLA typing of large numbers of donors is often a requirement for large bone marrow registries. The SSOP technique also utilizes the ‘patchwork of …