Homology-directed repair (HDR) is a mechanism in cells to repair double-strand DNA lesions. [1] The most common form of HDR is homologous recombination. The HDR mechanism can only be used by the cell when there is a homologous piece of DNA present in the nucleus, mostly in G2 and S phase of the cell cycle. Other examples of homology-directed ...

How does homology directed repair fix DNA double-strand breaks? In eukaryotic cells, repair of DSBs occurs primarily by two pathways: Non-Homologous End-Joining (NHEJ) and Homology Directed Repair (HDR).

Jul 20, 2023 · Homology-directed repair (HDR), a method for repair of DNA double-stranded breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA donors, but remains...

Sep 27, 2024 · CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating precise site-specific DNA insertions, deletions, and substitutions. These precise edits are made possible through the use of exogenous donor templates that carry the desired sequence.

In the world of CRISPR/Cas9-based gene editing, HDR and NHEJ are two fundamental DNA repair pathways, each offering unique advantages. NHEJ provides speed and efficiency, making it ideal for creating knockouts, while HDR offers the precision needed for complex genetic modifications like knockins.

Dec 10, 2024 · CRISPR-Cas9-mediated homology-directed repair (HDR) is a versatile platform for creating precise site-specific DNA insertions, deletions, and substitutions. These precise edits are made possible through the use of exogenous donor templates that carry the desired sequence.

Jun 26, 2023 · Briefly, HDR is the process of copying a donor strand of DNA to the place where a DSB occurred. If the homologous DNA sequence that is being used as a template is identical to the original DNA sequence, then no errors should occur as a result of the repair process.

HDR is one of the two major DNA pathways for DNA damage repair. It requires a homologous template for restoration which may be provided endogenously or exogenously. HDR may be used for adding or removing specific DNA sequences at the location of the DSBs.

Jul 30, 2019 · Precise editing of genomic DNA can be achieved upon repair of CRISPR-induced DNA double-stranded breaks (DSBs) by homology-directed repair (HDR). However, the efficiency of this process is...

CRISPR/Cas9-induced HDR represents a tool to modify genomic DNA in a precise and controlled manner. However, owing to the preference of mammalian cells for NHEJ over HDR, the efficiency of HDR is significantly lower than that of NHEJ (25% vs. 75%).