H3K9ac is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the acetylation at the 9th lysine residue of the histone H3 protein. The H3K9 histone has two jobs. Genes get turned on if this mark is acetylated and silences them if methylated.

HDAC2 inhibition increased H3K9ac levels in prophase, whereas HDAC3 or SIRT1 inhibition increased H3K9ac levels in metaphase. Next, we performed ChIP-seq on mitotic-arrested cells following targeted inhibition of these histone deacetylases.

Feb 20, 2023 · Through Chromatin immunoprecipitation-sequencing (ChIP-seq) assay, we surprisingly found that tags of H3K9ac and H3K27ac on the promoter intensely decreased by 13.1% and 6.8%, respectively,...

May 10, 2022 · In this study, a genome-wide characterization for acetylated lysine 9 on histone H3 (H3K9ac) binding regions in normal temperature (18 °C) and heat-stress conditions (26 °C) via ChIP-seq were carried out. The results that revealed H3K9ac was an extensive epigenetic modulation in A. japonicus.

Dec 17, 2018 · We conducted an epigenome-wide association study using the histone 3 lysine 9 acetylation (H3K9ac) mark in 669 aged human prefrontal cortices; in contrast with amyloid-β, tau protein burden had...

Aug 24, 2012 · Genome-wide H3K9ac and H3K14ac show very high correlation between each other as well as with other histone marks (such as H3K4me3) suggesting a coordinated regulation of active histone marks.

To explore the function of histone methylation and acetylation in PaWB, we analyzed ChIP-Seq data for PF, PFI, PF-60 and PFI-60 samples using antibodies that recognize H3K4me3, H3K36me3 and H3K9ac. Statistical details of the ChIP and input sequencing data for each sample are listed in Table S2.

Jan 30, 2024 · Here, we profiled the genome-wide landscapes of H3K9ac for paired adjacent normal (Nor), primary ESCC (EC) and metastatic lymph node (LNC) esophageal tissues from three ESCC patients. Compared to...

Histone H3 acetyl Lys9 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT ® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 10 6 cell equivalents per ChIP) using 10 µl of Histone H3 acetyl Lys9 antibody or the equivalent amount of rabbit IgG as a negative control.