Guide RNA (gRNA) or single guide RNA (sgRNA) is a short sequence of RNA that functions as a guide for the Cas9 -endonuclease or other Cas-proteins [1] that cut the double-stranded DNA …

This year’s Nobel prize for the CRISPR/Cas system is an illustrative example of how scientific breakthroughs rests on preceding work: the discovery of guide RNAs in the 1990s. Subject …

Apr 30, 2022 · Guide RNA (gRNA): A specific RNA sequence that recognizes the region of interest in the target DNA. It binds with the Cas9 protein and directs it to the target site to …

Nov 5, 2020 · The team teased out some guidelines for guide RNA design, which led to their Rule Set 1 and Rule Set 2 — quantitative models to assess sgRNA on-target specificity.

Guide RNA refers to a RNA molecule that contains an anchor sequence and is used to direct the editing of preedited RNA by hybridizing with it and guiding the addition or deletion of …

Here, we review the state of the art in CRISPR gRNA design for research applications of the CRISPR‐Cas9 system, including knockout, activation, and inhibition. Notably, achieving good …

One area of focus in this optimization effort is the delivery of multiplexed single-guide RNAs (sgRNAs), the single-stranded RNAs that guide the Cas9 enzyme to specific DNA targets. …

Guide RNA is composed of two main parts: the scaffold RNA and the targeting RNA. The scaffold stabilizes the Cas9 enzyme, while the targeting RNA specifies the DNA sequence for …

Nov 6, 2023 · The primary function of guide RNAs lies in their ability to bind to complementary regions within the target RNAs, orchestrating the installation of specific modifications. Both …

Apr 11, 2025 · Here Ishikawa et al. determine the cryo-electron microscopy structures of the PspCas13b–guide RNA complex alone and in complex with target RNA, as well as that of the …