In genetic engineering, floxing refers to the insertion of a DNA sequence (which is then said to be floxed) between two LoxP sequences, creating an artificial gene cassette which can then be conditionally deleted (knocked out), translocated, or inverted in a process called Cre-Lox recombination. [1] .

Plasmid Lox-Stop-Lox TOPO from Dr. Tyler Jacks's lab contains the insert loxP-STOP-loxP and is published in Genes Dev. 2001 Dec 15. 15 (24):3243-8. This plasmid is available through Addgene.

We present a method for the generation of conditional gene repair mutations that employs a loxP -flanked (floxed) selectable marker and transcriptional/translational stop cassette (neostop) located within the first intron of a target gene.

5 days ago · This regular plasmid gene expression vector system utilizes the LoxP-Stop-LoxP (LSL) cassette to achieve Cre-mediated conditional activation of gene expression in mammalian cells and animals. The LSL cassette comprises a LoxP-flanked (aka floxed) triple repeat of the SV40 polyadenylation sequence.

Plasmid AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0 from Dr. David Anderson's lab contains the insert G-CaMP3 and is published in Nature. 2013 Jan 31;493 (7434):669-73. doi: 10.1038/nature11810. This plasmid is available through Addgene.

flox/-: 表示该基因座为杂合,一个等位基因带有flox位点,另一个为敲除状态。 flox/flox: 表示该基因座为双重flox,两个等位基因均带有flox位点。 Cre-lox系统 中,flox位点可以使表达Cre酶的组织或细胞特异性地将floxed基因删除。 注：在作者基础上进行了部分修改。

The LoxP-Stop-LoxP (LSL) system is a method to control transgenic expression at a defined time point. Within this system, transgene expression should only be initiated in the presence of the enzyme Cre recombinase [34], and Cre is able to excise the LoxP sites [35] that flank the stop sequence, allowing transgene expression to be initiated.

Mar 3, 2020 · Here, upon tamoxifen administration, the Cre recombinase mediates excision of a loxP-flanked STOP cassette preceding the reporter gene, which subsequently allows the reporter expression and irreversibly tags the cell and its progeny.

Plasmid pcDNA3.1_Floxed-STOP mCherry from Dr. Moritoshi Sato's lab contains the insert pcDNA3.1_Floxed-STOP mCherry and is published in Nat Chem Biol. 2016 Dec;12 (12):1059-1064. doi: 10.1038/nchembio.2205.

May 29, 2024 · 在敲入模式中，STOP序列（转录终止元件）阻止目的基因表达，Cre通过重组loxP位点将STOP序列切除后、目的基因得以顺利表达，即实现了基因敲入效果。