Dead Cell Apoptosis Kits with Annexin V for Flow Cytometry
The Dead Cell Apoptosis Kit with Annexin V FITC and PI detects PS externalization in apoptotic cells using recombinant annexin V conjugated to green-fluorescent FITC dye and dead cells using PI. Necrotic cells that stain with PI show red fluorescence.
The Dead Cell Apoptosis Kit with Annexin V FITC & Propidium Iodide for flow cytometry provides a rapid and convenient assay for apoptosis. The kit contains recombinant Annexin V conjugated to fluorescein (FITC Annexin V), as well as a ready-to-use solution of the red-fluorescent propidium iodide (PI) nucleic acid binding dye.
FITC Annexin V Apoptosis Detection Kit with PI - BioLegend
BioLegend's FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner.
FITC Annexin V Apoptosis Detection Kit I - BD Biosciences
Therefore, staining with FITC Annexin V is typically used in conjunction with a vital dye such as propidium iodide (PI) or 7-Amino-Actinomycin (7-AAD) to allow the investigator to identify early apoptotic cells (PI negative, FITC Annexin V positive).
Annexin V-FITC/PI Apoptosis Detection Kit - MedChemExpress
MCE Annexin V-FITC/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence(Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+ ...
Annexin V staining assay protocol for apoptosis | Abcam
Nov 10, 2021 · Discover the procedure for early detection of apoptosis using annexin V staining, and propidium iodide (PI), in our detailed step-by-step protocol.
Annexin V-FITC Apoptosis Staining / Detection Kit - Abcam
Annexin V-FITC Apoptosis Staining / Detection kit uses FITC labeled protein Annexin V that binds to the phosphatidylserine molecules on the apoptotic cell membrane, which is normally located on the inner side of a healthy cell, allowing the identification and quantification of early apoptotic cells using flow cytometry or a fluorescent microscope.
- Reviews: 3
Annexin V-FITC/PI Apoptosis Kit - Elabscience
Buy Annexin V-FITC/PI Apoptosis Kit from Elabscience with lower price, only 15-20 min hand-on time, ideal for early apoptosis detection and no fixation-related false positive.
Dec 20, 2018 · Analyze Annexin V-FITC binding by flow cytometry (Ex = 488 nm; Em = 530 nm) using FITC signal detector (usually FL1) and PI staining by the phycoerythrin emission signal detector (usually FL2).
Annexin V-FITC, the FITC-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy. Due to the loss of integrity of membrane, PI can enter late apoptotic or necrotic cells to stain DNA.
- Some results have been removed