Drosha is a Class 2 ribonuclease III enzyme [5] that in humans is encoded by the DROSHA (formerly RNASEN) gene. [6][7][8] It is the primary nuclease that executes the initiation step of miRNA processing in the nucleus. It works closely with DGCR8 and in correlation with Dicer.

Mar 30, 2025 · DROSHA (Drosha Ribonuclease III) is a Protein Coding gene. Diseases associated with DROSHA include Pineoblastoma and Hereditary Breast Ovarian Cancer Syndrome. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Translational Control.

DROSHA is the catalytic subunit of the Microprocessor complex, which initiates microRNA (miRNA) maturation in the nucleus by recognizing and cleaving hairpin precursors embedded in primary transcripts. However, accumulating evidence suggests that ...

Drosha is the catalytic center of the microprocessor and has two RNase-III domains that make up a catalytic site with each domain making one of the two cuts necessary to excise the pre-miRNA. Drosha excises the pre-miRNA from the pri-miRNA by cutting at the base of the dsRNA stem–loop of the pre-miRNA [82].

Apr 29, 2016 · A recent study by Kwon et al. in Cell reveals the structure of a DROSHA construct in complex with the C-terminal region of DGCR8, thereby unveiling the topology and interactions between...

Jul 20, 2012 · DROSHA is a nuclear RNase III enzyme responsible for cleaving primary microRNAs (miRNAs) into precursor miRNAs and thus is essential for the biogenesis of canonical miRNAs.

Jan 14, 2016 · The heretofore-elusive structure of DROSHA provides insights into how nascent microRNAs are recognized and cleaved in their initial processing step and reveals an unexpected similarity between DROSHA and DICER, the enzyme …

Dec 12, 2013 · Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription-dependent manner.

Nov 25, 2021 · We recently discovered that a human RNase III, DROSHA, exhibits a single cleavage on the one strand of primary microRNAs (pri-miRNAs). This study revealed that DROSHAs from the other animals,...

Nov 24, 2014 · The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003). By database searching and screening of a human liver cDNA library, Wu et al. (2000) cloned a …