为助力免疫机制研究，上海南模生物自主研发一系列免疫研究相关的DTR小鼠，包含 B 细胞、T 细胞、NK 细胞、Treg 细胞等等特异性的 DTR 小鼠。 此外研发了条件性表达的 iDTR 小鼠 ，通过和特异性 Cre 小鼠以及 Dre 小鼠交配，能实现更多类型的细胞特异性剔除。

Diphtheria toxin A (DTA), a segment of the diphtheria toxin (tox), inhibits protein synthesis in cells. When released from a cell, DTA is nontoxic and cannot enter other cells independently ...

We will organize this by categories of DTR/DTA based models, Cre strains, and transcription factor knockout mice. In the next section we will discuss the discoveries made with these models regarding the functions of DCs in various immune responses.

Using different Dre- and Cre-expressing mouse lines, we showed that the surrogate gene reporters tdTomato and DTR were simultaneously expressed in target cells and in their descendants, and we observed efficient ablation of tdTomato …

One of the most popular ablation strategies involves transgenic expression of a primate diphtheria toxin receptor (DTR) on murine cells that are otherwise resistant to the bacterial exotoxin. The efforts of many laboratories using the DTR approach over the years have yielded numerous valuable insights into specific cell functions.

Recombinase responsive mouse lines expressing diphtheria toxin subunit A (DTA) are well established tools for targeted ablation of genetically defined cell populations.

此方法多用于研究特定细胞类群的功能，利用白喉毒素 (Diphtheria toxin, DT) 及其细胞表面的白喉毒素受体 (DT receptor, DTR) ，阻断细胞蛋白质的合成，最终导致细胞死亡。 在自然界中，DTR只在灵长类 (比如人，猴等) 动物中表达，而小鼠等啮齿类动物则由于没有DTR而对白喉毒素具有抵抗力。 因此，要实现特异性剔除一类细胞，需要先通过基因修饰技术使小鼠条件性表达DTA (DT subunit alpha gene) 或DTR，再配合Cre品系共同使用。 常用的方法有以下两种： 一种是将表 …

May 20, 2005 · A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin (DT).

简而言之，DTR小鼠就像一台精密的“细胞清除机器”，可以帮助研究者探索表达特定基因的一类细胞在体内具体的作用和功能。 在此基础上，报告基因EGFP (Enhanced green fluorescent protein) 和荧光素酶（Luciferase）的引入可以显著增强实验的可视化和定量分析能力。 通过这种策略，研究人员可以直接观察表达DTR基因的特定细胞或组织在小鼠体内的动态变化和功能。 并且通过体内成像技术（如生物发光成像），研究人员可以在活体小鼠中非侵入性地监测特定基因的表达和 …

A new system for lineage ablation is based on transgenic expression of a diphtheria toxin receptor (DTR) in mouse cells and application of diphtheria toxin (DT). To streamline this approach, we generated Cre-inducible DTR transgenic mice (iDTR) in which Cre-mediated excision of a STOP cassette rende …