We demonstrate that neural progenitor cells (NPCs) and immature dentate granule cells (DGCs) within the adult murine hippocampus are particularly sensitive to rAAV-induced cell death.

Jul 30, 2024 · The right hemisphere dentate gyrus (DG) was labeled with an AAV vector that expressed the jRGECO1a genetically encoded calcium sensor 36 under the control of the CaMKIIα promoter (DJ serotype AAV...

Identification of a DG granule-cell-selective AAV targeting approach. Selective viral targeting of distinct excitatory cell classes in the cortex and hippocampus is currently limited. The most widely used excitatory neuron promotor (i.e., CaMKIIα) induces transgene expression across most excitatory neuron classes in these regions.

Nov 2, 2017 · The quiescence of adult neural stem cells (NSCs) is regulated by local parvalbumin (PV) interneurons within the dentate gyrus (DG). Little is known about how local PV interneurons communicate with distal brain regions to regulate NSCs and hippocampal neurogenesis.

To do so, we made use of adeno-associated viral (AAV) expressing short hairpin RNA (shRNA) targeting TRPC6 into the hippocampal DG of male mice. We evaluated the TRPC6 silencing effect using a battery of cognitive behavioral examinations linked to hippocampus.

Jul 14, 2021 · We demonstrate that neural progenitor cells (NPCs) and immature dentate granule cells (DGCs) within the adult murine hippocampus are particularly sensitive to rAAV-induced cell death.

Enhancer adeno-associated viruses (enhancer-AAVs), a more versatile and translatable cell-specific vector targeting method, are garnering increasing interest in neuroscience. However, enhancer-AAVs with selec-tivity for distinct hippocampal principal neurons are currently limited.

Jun 11, 2023 · A flexible AAV tool capable of accurately and efficiently targeting this region is highly demanded. To fill this gap, we generate an AAV expressing Cre driven by the mini Map3k15 promoter, AAV/M1-Cre, which can be easily utilized to help tracing and manipulating CA2 pyramidal neurons.

May 6, 2022 · Our study identifies a key hypothalamic circuit that couples novelty signals to the production and maturation of ABNs, and highlights the activity-dependent contribution of circuit-modified ABNs...

Oct 28, 2020 · Here, we report a versatile pipeline that enables the direct side-by-side comparison of pre-selected AAV capsids in high-throughput and in the same animal, by combining DNA/RNA barcoding with...