Cyanine5 (Cy5) is a bright, far-red-fluorescent dye with excitation suited for the 633 nm or 647 nm laser lines and is used for imaging, flow cytometry and genomics.

Cy5 has a long tail going all the way into the UV. We have data from irradiation of Cy5-labeled DNA with UV-300 nm supplied by a 200 mW UV-LED at 6V (saturation), focused into a PCR tube.

Cy5.5 is a near-infrared (IR) fluorescence-emitting dye (excitation/emission maximum 678/694 nm). Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

Aug 25, 2021 · We demonstrate that the potentially deleterious photoconversion, however, can be exploited to develop a new photoactivation method for high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives.

Dec 4, 2009 · While we have shown previously that reactivation of the cyanine dye by visible light can be facilitated by a nearby chromophore that efficiently absorbs the activation light, the observation of UV absorption by dark-state Cy5 prompted us to try its direct reactivation with UV light without an assisting chromophore.

Sep 27, 2024 · Cy5® NHS Ester (GE Healthcare) and Cyanine5 NHS ester are chemically equivalent, far-red fluorescent dyes used for labeling peptides, proteins and oligonucleotides, with an excitation wavelength of 651 nm and an emission wavelength of 670 nm.

Mar 31, 2016 · We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated...

Type: Near-infrared (NIR) fluorescent dye molecule commonly used in bioconjugation for applications like microscopy, flow cytometry, and in vivo imaging. High brightness: Offers strong fluorescence intensity for sensitive detection. Convenient conjugate labeling: Can be readily attached to biomolecules like proteins and antibodies.

We report the photo-induced nucleation and growth of silver nanoparticles in aqueous solution in the presence of DNA oligomers. An organic dye (Cy5) was used as a photosensitizer to initiate the...

Cy5 is particularly valued for its high fluorescence intensity and photostability, making it an excellent choice for sensitive applications such as fluorescence microscopy, flow cytometry, and fluorescence-based assays.