CUT&Tag-sequencing combines antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global DNA binding sites precisely for any protein of interest.

Apr 29, 2019 · Here we describe Cleavage Under Targets and Tagmentation (CUT&Tag), an enzyme-tethering strategy that provides efficient high-resolution sequencing libraries for profiling diverse chromatin...

Oct 7, 2024 · Here, we develop scNanoSeq-CUT&Tag, a streamlined method, by adapting modified cleavage under targets and tagmentation (CUT&Tag) to the nanopore sequencing platform for genome-wide chromatin...

Mar 13, 2020 · CUT&Tag insert libraries with the sequence of adapters. Our standard pipeline is to perform single-index 25x25 PE Illumina sequencing on up to 90 pooled samples on a single HiSeq 2500 flowcell, where each sample has a unique PCR primer barcode.

Mar 27, 2025 · Cleavage Under Targets & Tagmentation (CUT&Tag) is an enzyme-tethering approach that has been presented as a streamlined, easily scalable, and cost-effective alternative to ChIP-seq. CUT&Tag has ...

2 days ago · Like CUT&RUN, Cleavage Under Targets & Tagmentation (CUT&Tag) is a faster, more cost-friendly alternative to ChIP-seq. Compared to ChIP-Seq, CUT&Tag profiles chromatin with less sample, a simplified workflow, lower sequencing costs, and improved signal-to-noise due to lower backgrounds.

Nov 24, 2020 · CUT&Tag, which is short for Cleavage Under Targets and Tagmentation, is a molecular biology method that researchers use to investigate interactions between proteins and DNA and to identify DNA binding sites for their protein of interest.

Oct 10, 2022 · CUT&Tag bypasses the most challenging and time-consuming parts of ChIP-seq assays, including cross-linking, chromatin fragmentation, and immunoprecipitation (IP). Eliminating these steps provides multiple advantages (Figure 1): Fewer cells.

To tackle this challenge, scientists at Peking University have developed a new method called scNanoSeq-CUT&Tag. This innovative approach adapts an existing technique known as CUT&Tag, which stands for “Cleavage Under Targets and Tagmentation.”

Dec 17, 2023 · Scalable single-cell CUT&Tag can be combined with distinct platforms to distinguish cellular identity, epigenetic features and even spatial chromatin profiling. In addition, CUT&Tag has been developed as a strategy for joint profiling of the epigenome, transcriptome or proteome on the same sample.