A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been developed and validated, for the quantitation of four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin) from dietary ingredients and supplements.

Using the UV7 Excellence spectrophotometer, cyanocobalamin identification is a simple, fast and efficient method, with automatic evaluation reducing the risk of error.

Vitamin B12 (cobalamin) plays a key role in human biological functions and is vital in the neurological development of infants. Vitamin B12 exists in different chemical forms (i.e.,...

• Standard practices identify and quantify bioactive forms of vitamin B12 from dietary supplements simultaneously by HPLC/UV-Vis. Additionally, Chromatographic performance at different wavelength are compared to determine optimal peak resolution for all four vitamin B12.

Jun 1, 2019 · A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been developed and validated, for the quantitation of four bioactive forms of vitamin B12 (adenosylcobalamin, cyanocobalamin, hydroxocobalamin, methylcobalamin) from dietary ingredients and supplements.

In this paper, a novel thermal-reduced carbon dots (t-CDs) based fluorescence resonance energy transfer (FRET) sensor for determination of vitamin B12 (VB12) in aqueous solution is reported.

Cyanocobalamin or vitamin B12 analysis is an application used in the food and beverage industry as well as in academia. It is an essential B vitamin and is classified as water-soluble. In this application note, vitamin B12 analysis is performed according to the USP 35 NF 30 S2 method using a UV7 Excellence spectrophotometer .

Dec 18, 2022 · The Y-CDs had a high quantum yield (15.9%) and demonstrated photostability at various pHs, temperatures, and ionic strengths. The Y-CDs could detect cobalamin effectively and selectively, showing a linear relationship between fluorescence intensity …

The UV-vis spectra of the vitamin B12, methylcobalamin and B12 coenzyme were recorded in the same spectral conditions. The method of central composite design was used in the ranges of 10-80 mg L (-1) for vitamin B12 and methylcobalamin and 20-130 mg L (-1) for B12 coenzyme.

Typical cobalamins are characterized by a visible absorption spectrum consisting of peaks labeled α, , and sh. The physical basis of these β peaks as having electronic origin or as a vibronic progression is ambiguous despite much investigation. Here, for the first time, cobalamin uorescence is identi ed in several fl fi derivatives.