Jan 12, 2017 · We use this "optoDroplet" system to study condensed phases driven by the IDRs of various RNP body proteins, including FUS, DDX4, and HNRNPA1. Above a concentration threshold, these constructs undergo light-activated phase separation, forming spatiotemporally definable liquid optoDroplets.

Jun 23, 2017 · Here, we identify important determinants for efficient cryptochrome 2 clustering and introduce a new CRY2 module, named ‘‘CRY2clust’’, to induce rapid and efficient homo-oligomerization of target...

Apr 12, 2021 · We propose that Cul4 COP1/SPAs and Cul3 LRBs E3 ligases interact with CRY2 via different structure elements to regulate the abundance of CRY2 photoreceptor under different light conditions,...

Jan 1, 2021 · Upon blue light illumination, Cry2-eYFP-VP16 (OptoTF-) is recruited to the promoter via heterodimerization of Cry2 and CIBn. Homomultimerization of Cry2 leads to further accumulation of the TAD VP16, resulting in high expression of the reporter gene.

Photoactivated CRY2 forms photobodies through phase separation (Wang et al., 2021). The CRY2 C‐terminal extension (CCE) domain and its phosphorylation are required for maintaining the liquid property of CRY2 photobody (Wang et al., 2021). However, the residues essential for CRY2 phase separation and the underlying mechanism remain elusive.

Mar 31, 2023 · Here, we devised a biotinylated CRISPR-dCas9–based optogenetic method, light-activated macromolecular phase separation (LAMPS), to enable inducible formation, affinity purification, and multiomic dissection of nuclear condensates at the targeted genomic loci.

Nov 29, 2018 · Here, we use CasDrop, a novel CRISPR-Cas9-based optogenetic technology, to show that various IDPs phase separate into liquid condensates that mechanically exclude chromatin as they grow and preferentially form in low-density, largely euchromatic regions.

May 22, 2020 · The discovery of selective modulators of two Cryptochrome isoforms, CRY1 and CRY2, permits a deeper understanding of how circadian clock proteins impact diverse aspects of our daily 24-h rhythms...

Dec 24, 2024 · CRY2 (Cryptochrome Circadian Regulator 2) is a Protein Coding gene. Diseases associated with CRY2 include Advanced Sleep Phase Syndrome and Delayed Sleep Phase Disorder. Among its related pathways are Metabolic States and Circadian Oscillators and Circadian Clock.

Jan 12, 2017 · For IDR-fusion Cry2 plasmids, DNA fragments encoding the IDRs were inserted into the linearized pHR-mCh-Cry2WT (or Cry2olig) backbone using In-Fusion Cloning Kit (Takara). The resulting constructs were fully sequenced to confirm the …