Jan 11, 2024 · In liquid chromatography (LC), the mobile phase is a liquid in which the sample with ions and molecules is dissolved. The 2 most common forms of LC used for analysis are liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid …

Understanding the attributes of both the mobile phase and stationary phase is essential for optimizing chromatographic separations. In this article, we will explore and compare the key characteristics of these two phases.

Jan 1, 2023 · To get the process started, the mixture is dissolved in a substance called the mobile phase, which allows it to move through a second substance called the stationary phase. Chromatography was initially used by artists, color theorists, and artisans, hoping to perfect industrial dyes for textiles.

Jan 24, 2023 · In chromatography we pass a sample-free phase, which we call the mobile phase, over a second sample-free stationary phase that remains fixed in space (Figure \(\PageIndex{1}\)). We inject or place the sample into the mobile phase.

Liquid chromatography (LC) separates molecules in a liquid mobile phase using a solid stationary phase. Liquid chromatography can be used for analytical or preparative applications. Here, we restrict our discussion to column liquid chromatography techniques and considerations.

In reversed-phase chromatography, which is the more common form of HPLC, the stationary phase is nonpolar and the mobile phase is polar. The most common nonpolar stationary phases use an organochlorosilane where the R group is an n -octyl (C 8 ) or n -octyldecyl (C 18 ) hydrocarbon chain.

Dec 28, 2024 · Mobile Phase: The phase that moves through the chromatographic system, transporting the sample. Stationary Phase: The phase that remains fixed in place and interacts with the sample components, causing them to separate.

Oct 17, 2019 · Chromatography is a process for separating components of a mixture. To get the process started, the mixture is dissolved in a substance called the mobile phase, which carries it through a second substance called the stationary phase.

Jan 1, 2023 · This chapter summarizes the most common strategies used by experienced chromatographers in reversed-phase, normal-phase, and hydrophilic interaction liquid chromatography for mobile phase selection.

Apr 16, 2024 · Chromatography is a method by which a mixture is separated by distributing its components between two phases. The stationary phase remains fixed in place while the mobile phase carries the components of the mixture through the medium being used.