Jul 1, 2016 · The direct measurement of drug-protein interactions in living cells is a major challenge in drug discovery research. Using the cellular thermal shift assay or CETSA such …

CEllular Thermal Shift Assay (CETSA®) is a patented label free chemoproteomics method that has enabled measurements of compound target engagement in intact cells and tissue, without …

Aug 7, 2014 · The cellular thermal shift assay–CETSA–exploits this to characterize intracellular drug-protein engagement. Thermal shift assays are used to study thermal stabilization of …

The CETSA protocol consists of four key steps: compound incubation with live cells or lysates, heat treatment at different temperatures, separation of proteins that remain folded from the …

Jul 5, 2013 · We have developed a method for evaluating drug binding to target proteins in cells and tissue samples. This cellular thermal shift assay (CETSA) is based on the biophysical …

CETSA is a biophysical assay based on the principle of ligand-induced thermal stabilization of target proteins, meaning that a proteins melting temperature will change upon ligand interaction.

Jul 5, 2013 · We have developed a method for evaluating drug binding to target proteins in cells and tissue samples. This cellular thermal shift assay (CETSA) is based on the biophysical …

Jan 1, 2020 · The Cellular Thermal Shift Assay (CETSA®) is well suited for identifying target engagement between ligands and their protein targets. Several studies have shown that …

Here, we present the development and validation of a homogeneous, standardized, target-independent, and high-throughput (384- and 1536-well formats) CETSA platform that uses a …

Feb 8, 2025 · Cellular Thermal Shift Assay (CETSA) is a valuable technique for studying protein stability and interactions within cells. It plays a crucial role in drug discovery by validating …