Cell line development (CLD) is the engineering of a cell line, often mammalian, to produce a therapeutic biomolecule or biologic. It allows scientists to tailor cultures depending on the applications and desired properties of the targeted product.

As such, the cell line development process typically requires 6 to 12 months and is a time, capital and labour intensive process. This article reviews established advances in protein expression and clone screening which are the core technologies in mammalian cell line development.

Cell line development is the process by which living cells are used to manufacture therapeutic biologics or other proteins of interest. Cell line development starts at small-scale suspension cultures, eventually transitioning to industrial-scale bioreactors at the manufacturing stage.

Discover the key phases of cell line development from antibody production to master cell banking and the solutions we offer for each stage of the journey. Read and download free resources to learn how to streamline your cell line development workflow.

Cell line development (CLD) is the process of bioengineering cells or cellular expression systems to stably express and manufacture biotherapeutics or biological products such as vaccines, monoclonal antibodies, recombinant proteins and enzymes.

Jan 1, 2018 · In this chapter, we describe how such stable CHO cell lines can be developed, propose options for an optimized cell line development process and discuss host cell engineering strategies and biosafety analysis.

Jun 21, 2024 · Cell line development is the process of creating a stable cell line that can produce a specific therapeutic protein or biomolecule at high levels. This involves the genetic modification of cells, often mammalian cells, to incorporate the gene of interest, followed by rigorous selection and screening to ensure that the cells can consistently and ...

With ATUM's Leap-In technology, cell line development is less labor intensive and does not require automation. No matter how small your team, many projects can be conducted simultaneously, from cell line development to cell line generation and cell line engineering.

What is stable cell line development? Stable cell line development involves introducing and verifying the stable genetic integration, growth and expression of cell lines producing target proteins or biologics.

This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis.