Kompetitive allele specific PCR (KASP) is a homogenous, fluorescence-based genotyping variant of polymerase chain reaction. It is based on allele-specific oligo extension and fluorescence resonance energy transfer for signal generation.

Nov 8, 2017 · Most CAPS markers are co-dominant and locus-specific. Most CAPS genotypes are easily scored and interpreted. CAPS markers are easily shared between laboratories. CAPS assay does not require the use of radioactive isotopes, and it is more amenable, therefore, to analyses in clinical settings. Sequence the RFLP probe.

Kompetitive Allele Specific PCR (KASP™) assays enable accurate, bi-allelic discrimination of SNPs and indels. Unmatched quality, reliability and credibility have established KASP as a foundational SNP genotyping chemistry for agrigenomics.

The cleaved amplified polymorphic sequence (CAPS) method is a technique in molecular biology for the analysis of genetic markers. It is an extension to the restriction fragment length polymorphism (RFLP) method, using polymerase chain …

The KASP genotyping assay utilizes a unique form of competitive allele-specific PCR combined with a novel, homogeneous, fluorescence-based reporting system for the identification and measurement of genetic variation occurring at the nucleotide level to detect single nucleotide polymorphisms (SNPs) or inserts and deletions (InDels).

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs [4] have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts.

Apr 15, 2024 · KASP is an allele-specific oligo extension-based PCR assay that uses fluorescence resonance energy transfer (FRET) to detect genetic variations such as SNPs and insertions/deletions (InDels) at a specific locus.

This tool uses primer3 to design KASP primers for given flanking sequences of SNPs/indels. If the genome of your species has gene duplicates (such as wheat), please try SNP_Primer_Pipeline2 for gene/sub-genome specifc primers. Input file is a text file with each line looks like below (at least 50bp on each side of the SNP).

Jun 30, 2023 · Molecular marker-assisted breeding techniques enable plant breeders to quickly and accurately identify and enhance the traits of interest. KASP, or competitive allele-specific PCR, is the most recent development in the field of molecular markers-aided breeding technology.

The EiB low-density genotyping service is based on KASP markers. Kompetitive Allele Specific PCR (KASP) is a simplified fluorescence-based methodology to genotype specific polymorphisms or INDELS.