Caco-2 (from Cancer coli, "colon cancer") is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. [1] In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells. [1] It was developed in 1977 by Jorgen Fogh ...

Caco-2 [Caco2] is an adherent cell line isolated from colon tissue derived from a patient with colorectal adenocarcinoma. These cells express characteristics of enterocytic differentiation upon confluence and are a commonly used line for mimicking the small intestine in …

The human epithelial cell line Caco-2 has been widely used as a model of the intestinal epithelial barrier. The Caco-2 cell line is originally derived from a colon carcinoma.

In this review, we presented permeability tests in the Caco-2 model used for both scientific (e.g., drug absorption prediction) and regulatory purposes (e.g., formal classification of substances into a BCS group).

The polarized Caco-2 cell layer shows 4 times higher TEER values compared to HT29 monolayers, i.e. more similar to the in vivo situation. Caco-2 cells express most receptors, transporters and drug metabolizing enzymes like aminopeptidase, esterase and sulfatase found in normal epithelium.

Caco-2, a cell line obtained from human colon adenocarcinoma, is the most well-established intestinal cell model to date. Caco-2 cells differentiate into enterocyte-like cells after 21 days in culture and express a number of characteristic transporters [1,2]. Since Caco-2 is the gold-standard model to study intestinal absorption, this review ...

This novel in vitro Caco-2-based mucosal model can be used to study intestinal barrier functions and microbe–mucus interactions. The Caco-2 cells were grown on Transwell plates in low-glucose medium.

Although the colonic cell line Caco-2 is widely used as a model of the small intestinal barrier function, it has limitations such as overestimated transepithelial electrical resistance (TEER) compared to in vivo conditions. Therefore, we investigated Human Intestinal Epithelial Cells (HIECs) as an alternative in vitro model.

Epithelial properties of human intestinal Caco-2 cells cultured in a serum-free medium. Neoplastic progression of human and rat intestinal cell lines after transfer of the ras and polyoma middle T oncogenes.

Aug 25, 2022 · The Caco-2 cell model has been widely used to assess the permeability of drug candidates. It has provided a high throughput in vitro platform, functionally resembling the enterocytes.