CHO-K1 cells also do not express EGFR. CHO-DG44 cells were derived from the original CHO-K1 cells by several rounds of mutagenesis that deleted both copies of the dhfr genes. Dihydrofolate reductase (dhfr) is required for the de novo synthesis of purines, thymidylic acid, and certain amino acids and is required for growth and proliferation of ...

Jun 3, 2013 · Dr. Theodore Puck received a female Chinese Hamster from a laboratory at the Boston Cancer Research Foundation and used the animal to derive the original cell lines. One line was known as CHO-K1, was obtained from those first CHO lines. CHO-K1 is an adherent line and was traditionally grown of F12 Medium supplemented with 10% FBS.

Oct 25, 2022 · For example, Efficient-Pro™ Feed 1 is designed for CHO-K1 and CHO-K1 GS cell lines, while Efficient-Pro™ Feed 2 is optimized for CHO-S, DG-44, and CHOTZN™ lines. These combinations of medium and feeds are formulated to support most formats and cell types resulting in titers above 4mg/mL and high specific productivity.

Dec 15, 2016 · The MaxCyte electroporation-based platform is flexible able to efficiently transfect a variety of CHO cell lines including CHO-S, CHO-K1, CHO EBNA, CHO-K1SV, and CHOZN®. High titers, consistent protein quality and reproducibility were demonstrated when compared with other transfection methods (Figure 1, 2)

CHO-K1: MaxCyte STX Outperforms Other Methods 3-24 Fold Higher Protein Titers than Variety of Other Methods Figure 2: Scale up of CHO-S Cells from Small to Large Scale Using the MaxCyte Platform. Two sets of 2E10 CHO-S cells were transfected by flow EP with an hIgG expression plasmid using the MaxCyte STX and VLX instruments.

Apr 4, 2022 · Gibco™ Efficient-Pro™ Feed 1 is optimized for CHO-K1 cell lines, while Gibco™ Efficient-Pro™ Feed 2 is best suited for CHO-S and CHO-DG44 cell lines. This complete workflow solution is designed to provide greater volumetric and specific productivity, higher titers, and enhanced protein quality through improved charge variant and glycan ...

Feb 9, 2021 · CHOSOURCE™ GS Knockout Cell Line Provides Flexible Solutions to Biomanufacturing Challenges. February 09, 2021 • Written by Brandy Sargent

Feb 10, 2016 · CHO Media Development and Optimization – A look at three case studies. February 10, 2016. CHO media development and optimization is always a popular topic since media plays a key role in biomanufacturing success.

Mar 9, 2021 · We then discussed how from the original isolation of the CHO-K1 cells, many derivatives have emerged over the years; CHO-S, CHO-DG44, CHO-K1 variants, and more recently knockouts to improve clonal selection or eliminate undesirable enzymatic activities.

May 21, 2024 · The tool further offers a selection of recommended Thermo Fisher, Nunc, Invitrogen, and Gibco products that have undergone validation for use with the CHO-K1 cell line. Covering everything from basal media to various FBS products, the tool provides a comprehensive product list, which simplifies the product selection process and makes it easy ...