The immediate early gene c-fos has long been known as a molecular marker of neural activity. The neuron’s activity is transformed into intracellular calcium influx through NMDA receptors and L-type voltage sensitive calcium channels. For the ...

Nov 23, 2015 · Membrane depolarization, brain-derived neurotrophic factor (BDNF) and forskolin activate distinct subsets of the enhancers to induce c-fos transcription in neurons, suggesting that...

Reporters of c-fos allow detection of transcriptional activation even in deep nuclei, without requiring the accurate targeting of multiple electrodes at long distances.

Mar 4, 2002 · Emerging evidence suggests that c- fos is essential in regulating neuronal cell survival versus death 10. Although c- fos is induced by neuronal activity, including kainic acid-induced...

c-Fos is a 380 amino acid protein with a basic leucine zipper region for dimerisation and DNA-binding and a transactivation domain at C-terminus, and, like Jun proteins, it can form homodimers. [8] . In vitro studies have shown that Jun–Fos heterodimers are more stable and have stronger DNA-binding activity than Jun–Jun homodimers. [9]

Transcription of c-fos occurs within minutes of application of a stimulus, amounts of mRNA peak at 30–45 min, and the half-life of the Fos protein is about 2 h (Muller et al., 1984). The expression of c-fos in neurons has provided a useful and popular marker of activated neurons.

Calcium fluxes into the neuron during action potentials via NMDAr and voltage-dependent calcium channels. The influx of calcium binds calmodulin, activating CaMK II or IV and then CREB. NMDAr activity also activates RasGRF1, activating the …

Jun 22, 1990 · C-fos is a proto-oncogene that is expressed within some neurons following depolarization. The protein product, c-fos protein, can be identified by immunohistochemical techniques. Therefore, c-fos expression might be used as a marker for neuronal activity throughout the neuraxis following peripheral stimulation.

It is a kind of proto-oncogene that is expressed on external stimulation of cells with substances like transmitters, hormones, and neurotrophic factors, and that does not require synthesis of new proteins for its expression. The expression level of c-fos …

Membrane depolarization, brain-derived neurotrophic factor (BDNF), and Forskolin activate distinct subsets of the enhancers to induce c-fos transcription in neurons, suggesting that stimulus-specific combinatorial activation of multiple enhancers underlies the broad inducibility of …