Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene.

Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry. CD107a is significantly upregulated on the surface of NK cells following stimulation with MHC devoid targets.

In this chapter, we detail the steps required to isolate peripheral blood mononuclear cells (PBMCs), coculture them with target tumor cell lines, and evaluate the cytotoxic immune function by means of flow cytometry evaluation of CD107a expression on the surface of NK cells.

Studies show that CD107a (LAMP-1) may be a marker for degranulation of NK and activated CD8+ T cells. In our study, the relationship between the expression of CD107a, cytokine secretion and cytotoxic activity in CD56+ NK, CD8+ T cells and …

Aug 22, 2013 · In particular, CD107a/LAMP-1 has been widely used as a marker for degranulating cytotoxic lymphocytes, because surface expression is induced upon degranulation. 22-24 CD107a is a highly glycosylated protein comprising a 40-kDa backbone with predicted 17 N -glycosylation and 9 O -glycosylation sites.

Jun 1, 2017 · Perforin then forms a porelike structure on the membrane of the target cell, through which granzymes pass to mediate target cell death. CD107a is found within the membrane of cytolytic vesicles, and its expression increases on the surface of the NK cell as a consequence of cytolytic granule release.

Jun 6, 2013 · Lysosome-associated membrane protein (LAMP) 1/CD107a is used as a marker for NK-cell degranulation, but its role in NK-cell biology is unknown. We show that LAMP1 silencing causes inhibition of NK-cell cytotoxicity, as LAMP1 RNA interference (RNAi) cells fail to deliver granzyme B to target cells.

The results of this study reveal that impaired CD107a expression and IFN-γ production as cytotoxic response to CMV fails to be a major contributing factor for RSA. Cytotoxic responses to different antigens or different component of CMV virus may have to be used in future studies.

Nov 1, 2004 · Recently, lysosomal-associated membrane protein-1 (LAMP-1 or CD107a) has been described as a marker of CD8 + T-cell degranulation following stimulation. Here we describe CD107a as a marker of NK cell functional activity using multi-parameter flow cytometry.

NK cell function testing is less sensitive and no more specific for discriminating genetic HLH compared to perforin and CD107a expression. Perforin and CD107a testing could augment NK-cell cytotoxicity testing for use in HLH diagnostic criteria.