Sep 3, 2024 · In B16 melanoma and MC38 colon cancer, LDH inhibition resulted in increased glucose-uptake capacity in all 3 T cell subtypes, suggesting potentiation of antitumor Teff function and reduction of Treg-mediated immunosuppression.

Mar 19, 2025 · Whereas control cells were not susceptible to IFNγ-induced cell death, VDAC2-deficient B16-OVA cells showed markedly increased cell death and lactate dehydrogenase (LDH) release after IFNγ (but...

Lactate induces expression of programmed cell death protein-1 ligand (PD-L1) on B16 melanoma cells and deletion of lactate dehydrogenase-A (LDH-A) in B16 cells or anti-PD-1 therapy prevents tumor growth.

Sep 3, 2024 · Our results establish LDH inhibition as an effective strategy for rebalancing glucose availability for T cells within the TME, which can enhance T cell function and antitumor immunity.

By characterizing the metabolic effects of IL-2 versus IL-21, a closely related cytokine that expands cells but lacks the detrimental effects of IL-2, we identified a pivotal role of lactate dehydrogenase (LDH) in regulating CD8 + T cell effector differentiation.

Feb 26, 2010 · Quantification of lactate dehydrogenase (LDH) is a well-established assay for cell viability that provides fast, robust and reproducible insights into the potential toxicity of experimental compounds and potential drug candidates.

Here we report that only the combined genetic disruption of LDHA and LDHB will fully suppress lactate secretion with a concomitant reduction in glucose consumption by the human colon adenocarcinoma cell line LS174T and the murine melanoma cell line B16-F10.

Nov 5, 2024 · We injected syngeneic hosts with B16-F10 melanoma expressing OVA (B16-OVA), then transferred OTI or OTI/LDH-A-/-T cells and monitored tumor growth. Strikingly, we found significantly larger tumors, a higher CD4/8 T cell ratio, and less CD8 + T cell infiltration in recipients of OTI/LDH-A-/-T cells.

Nov 5, 2024 · We injected syngeneic hosts with B16-F10 melanoma expressing OVA (B16-OVA), then transferred OTI or OTI/LDH-A-/-T cells and monitored tumor growth. Strikingly, we found significantly larger tumors, a higher CD4/8 T cell ratio, and less CD8 + T cell infiltration in recipients of OTI/LDH-A-/-T cells.

Sep 3, 2024 · LDH inhibition improves antitumor T cell functions. (A) Schematic depicting tumor-killing assay with LDHi in which B16-YFP cells were treated with 20 μM LDHi or vehicle 24 hours apart and T cells...