Dec 18, 2009 · By studying co-expressed M23 and M1 cysteine mutants here, we found that M1 palmitoylation does significantly alter AQP4 assembly at physiological temperature, not by altering the ability of M1 alone to form OAPs but, instead, by regulating the formation of …

Aquaporin-4 (AQP4) water channels exist as heterotetramers of M1 and M23 splice variants and appear to be present in orthogonal arrays of intramembraneous particles (OAPs) visualized by freeze-fracture microscopy.

We hypothesize a role of the M23-AQP4 in the regulation of M1-AQP4 expression. M1-AQP4 transcription, splicing and M1-AQP4 protein degradation were found to be unaffected in M23-null spinal cord and in M23-null astrocyte primary culture.

Jul 29, 2014 · NMO-IgG/AQP4-Ab seropositivity in patients with NMO and its spectrum disorders has important differential diagnostic, prognostic and therapeutic implications. So-called cell-based assays (CBA) are thought to provide the best AQP4-Ab detection rates.

Feb 2, 2014 · Here, we demonstrate that the AQP4 aggregation state determines its subcellular localization and cellular functions. Individually expressed M1–AQP4 was freely mobile in the plasma membrane and could diffuse into rapidly extending lamellipodial regions to …

The relevance of AQP4 square array assembly to the physiology and pathophysiology of brain is unknown. Our studies suggest that M1 and M23 may restrict AQP4 square array assembly to a preferred size, but it is not clear why this is important. AQP4 has a …

Jan 11, 2024 · Aquaporin-4 (AQP4) is a water channel protein found primarily in the central nervous system (CNS) that helps to regulate water–ion homeostasis. AQP4 exists in two major isoforms: M1 and M23.

By studying co-expressed M23 and M1 cysteine mutants here, we found that M1 palmitoylation does significantly alter AQP4 assembly at physiological temperature, not by altering the ability of M1 alone to form OAPs but, instead, by regulating the formation of mixed M1/M23 arrays.

May 9, 2011 · The two AQP4 isoforms produced by alternative spicing, M1 and M23 AQP4, form heterotetramers that assemble in cell plasma membranes in supramolecular structures called orthogonal arrays of ...

Dec 18, 2009 · Here, we studied M1/M23 interactions and regulated OAP assembly by nanometer-resolution tracking of quantum dot-labeled AQP4 in live cells expressing differentially tagged AQP4 isoforms and in primary glial cell cultures in which native AQP4 was labeled with a monoclonal recombinant neuromyelitis optica autoantibody.