In this study, we explored the use of vectors based on AAV6 to increase transduction rates in airways. AAV vectors were made using combinations of rep, cap, and packaged genomes from AAV2 or AAV6. The packaged genomes encoded human placental alkaline phosphatase and contained terminal repeat sequences from AAV2 or AAV6.

Eleven serotypes of AAV have thus far been identified, with the best characterized and most commonly used being AAV2. These serotypes differ in their tropism, or the types of cells they infect, making AAV a very useful system for preferentially transducing specific cell types.

Oct 19, 2016 · In our present studies, we describe two additive strategies to augment the transduction efficiency of AAV6 serotype vectors in primary human CD34 + cells, as well as propose a hypothetical model...

Oct 8, 2015 · In current studies, we show that AAV6 vectors can effectively transduce several distinct human cancer cell lines, such as prostate adenocarcinoma (PC3), mammary gland ductal carcinoma (T47D) and...

May 18, 2020 · Here, we describe an optimized Cas9-AAV6-based genome editing tool platform for site-specific mutagenesis and integration of up to more than 3 kilobases of exogenous DNA in the genome of hMSCs...

Adeno-associated virus serotype 6 (AAV6) is a valuable reagent for genome editing of hematopoietic cells due to its ability to serve as a homology donor template. However, a comprehensive study of AAV6 transduction of hematopoietic cells in culture, ...

AAV6 is missing the positively charged AAV2 R585 and R588 residues but compensates with two positively charged lysines at a different location (K459 and K493). Preliminary evidence suggested the AAV2 and AAV6 sites were more similar to each other compared to AAV2 and AAV3B HSPG attachment sites .

Nov 13, 2023 · Adeno-associated viruses (AAVs) are gaining interest in the development of cellular immunotherapy. Compared to other viral vectors, AAVs can reduce the risk of insertional oncogenesis. AAV serotype 6 (AAV6) shows the highest efficiency for transducing T cells.

Dec 30, 2020 · We find that AAV6 using a CAG promoter to drive transgene expression, delivered into the NP of murine caudal discs at a titer of 10 11 GC/mL, provides excellent transduction efficiency/kinetics and low toxicity in vivo.

Sep 10, 2021 · Adeno-associated virus serotype 6 (AAV6) is a valuable reagent for genome editing of hematopoietic cells due to its ability to serve as a homology donor template. However, a comprehensive study of AAV6 transduction of hematopoietic cells in culture, with the goal of maximizing ex vivo genome …