This permits the capture of unknown 3'-mRNA sequences that lie between the exon and the poly (A) tail. 5' RACE, or “anchored” PCR, is a technique that facilitates the isolation and characterization of 5' ends from low-copy messages.

Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR).

The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR.

Dec 29, 2006 · RACE (rapid amplification of cDNA ends) PCR is useful for quickly obtaining full length cDNAs for mRNAs for which only part of the sequence is known and to identify alternative 5′ or 3′...

In combination with LA PCR, several kilobases of unknown 5′-ends could be amplified. It is easy to perform, quick, inexpensive and reliable, which should enable it to replace most currently used 5′-RACE protocols.

Rapid amplification of cDNA ends (RACE) is a novel technique used to determine the 5′ end sequences of transcripts (2). In classic RACE, a homopolymer tail that acts as an anchor sequence can be added to the 3′ end of cDNA by terminal deoxynucleotidyl transferase (TdT).

The SMARTer RACE 5'/3' Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. First-strand cDNA generated with this kit can be used directly in 5'- and 3'-RACE PCR reactions.

The novel streamlined three-step 5′-RACE employs the following steps: (i) reverse transcription with 5′ phosphorylated gene-specific primers, (ii) cDNA circularization by RNL1 and (iii) PCR amplification using inverted primers followed by blunt end cloning for sequencing.

May 9, 2019 · Rapid amplification of cDNA ends (RACE) is a widely used technique to identify the 5′ (5′ RACE) or the 3′ (3′ RACE) end of an RNA transcript when its sequence is only partially known. This 5′ RACE protocol contains two steps.

5' RACE, or “anchored” PCR, is a technique that facilitates the isolation and characteri-zation of 5' ends from low-copy messages. The method has been reviewed by both Frohman (6,8) and Loh (7).