Dec 17, 2019 · Determining how chromosomes are positioned and folded within the nucleus is critical to understanding the role of chromatin topology in gene regulation. Several methods are available for...

Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.

May 3, 2019 · In this paper, we propose a divide-and-conquer based method, called GEM-FISH, which is an extended version of GEM 36 and an attempt to systematically integrate FISH data with both Hi-C data...

Jun 1, 2018 · We discuss the experimental design and provide practical considerations for 3D imaging and data analysis to determine chromatin folding. We demonstrate that 3D FISH using BACs (Bacterial Artificial Chromosomes) or fosmids can provide detailed information of the architecture of gene domains.

Jan 1, 2020 · Quantitative 3D image analysis of FISH labeled chromosome territories (Fig. 3) and gene domains revealed changes in radial positioning, sphericity, and orientation depending on the type and grade of tumors (e.g., in pancreatic tumors, cervix carcinomas, breast cancers etc.).

Fluorescence in situ hybridization (FISH) of specific DNA probes has become a widely used technique mostly for chromosome analysis and for studies of the chromosomal location of specific DNA segments in metaphase preparations as well as in interphase nuclei.

Fluorescence in situ hybridization (FISH) of specific DNA probes has become a widely used technique mostly for chromosome analysis and for studies of the chromosomal location of specific DNA segments in metaphase preparations as well as in interphase nuclei.

We present first results and assess the potential using 3D-SIM in combination with 3D fluorescence in situ hybridization (3D-FISH) for the topographical analysis of defined nuclear targets. Our study also deals with the concern that artifacts produced by FISH may counteract the gain in resolution.

Apr 9, 2019 · In the past decade, DNA FISH techniques have become increasingly popular among genome biologists, thanks to the fact that they enable direct observation of the three-dimentional (3D) genome...

DNA fluorescence in situ hybridization (DNA FISH) allows the three-dimensional visualization of individual gene loci, subchromosomal domains and even entire chromosomes during all stages of the cell cycle. 2D FISH is used for metaphase studies while 3D FISH has been extensively used to probe the relationship between the spatial organization of ...