May 30, 2022 · We set out to investigate whether BAP1 functions in the repair of UV-induced DNA damage. BAP1 depletion rendered 293T cells hypersensitive to UV-C light (254 nm, hereafter denoted UV) (Fig. 1A,...

The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. This cell line is competent to replicate vectors carrying the SV40 region of replication. It gives high titers when used to produce retroviruses.

For quantitative analysis of UV-induced RPA2 focus formation, the 293T or POLH-1 cells transfected with corresponding plasmids were treated with UV. Images were taken with the same exposure time.

Mar 7, 2019 · UV-induced mutation frequency is reduced to the same level in 293T cells depleted for XPA, Polθ, or for both XPA and Polθ (Figure S1D). The epistasis of Polθ with XPA is indicative of a role for Polθ in the aberrant XPA-dependent NER pathway.

The in vitro expression and electrophysiological recording of recombinant voltage-gated ion channels in cultured human embryonic kidney cells (HEK-293T) is a ubiquitous research strategy. HEK-293T cells must be plated onto glass coverslips at low ...

Mar 17, 2025 · We introduce a comprehensive approach to ultraviolet irradiation, including ultraviolet A/B and ultraviolet C, which enhanced the secretion of exosomes by HEK-293T targeted vectors for KRAS inhibition in pancreatic cancer.

May 31, 2022 · Making full use of lentiviral vector technology and the structure of the exosomal membrane, we developed an engineered HEK-293T exosome-based delivery system that intelligently integrates three functions: tumor targeting, 131 I and Dox coloading with high payloads, and tumor-targeted therapy.

Mar 15, 2025 · The 293T cell line possesses specific genetic alterations that distinguish it from its parental HEK 293 cells. These modifications improve transfection efficiency, facilitate viral vector production, and support high levels of recombinant protein expression.

Human embryonic kidney cells 293 (HEK-293) and 293T cells (those that contain SV40 Large T-antigen) show a reliable growth and have a propensity for transfection. Therefore, they are a major workhorse for research in cell biology.

Oct 1, 2013 · Furthermore, co-overexpression of Sef-S and TAK1 induce 293T cells apoptosis. These results reveal Sef-S actives Lys63-linked TAK1 polyubiquitination on lysine 209, induces TAK1-mediated JNK and p38 activation and also results apoptosis in 293T cells.