2-NBDG is a fluorescent tracer used for monitoring glucose uptake into living cells; it consists of a glucosamine molecule substituted with a 7-nitrobenzofurazan fluorophore at its amine group. It is widely referred to a fluorescent derivative of glucose, [1] and it is used in cell biology to visualize uptake of glucose by cells. [2]

2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ∼465/540 nm and can be visualized using optical filters designed for fluorescein.

2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry.

Among the most commonly used of these analogs are 2-NBDG and 6-NBDG, which contain a bulky 7-nitro-2,1,3-benzoxadiazol-4-yl-amino moiety in place of a hydroxy group on D-glucose. This fluorescent group significantly alters both the size and shape of these molecules compared to glucose, calling into question whether they actually enter cells by ...

In generating fluorescent glucose analogues, the nitrobenzoxydiazoamino group (NBDG) replaces a hydroxyl group (-OH) of a glucose molecule. In 2-NBDG, it is the -OH of carbon 2 that is replaced, whereas in 6-NBDG, the -OH of carbon 6 is replaced with NBDG.

Sep 30, 2005 · The assay is based on direct incubation of mammalian cells with a fluorescent d-glucose analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG) followed by flow cytometric detection of fluorescence produced by the cells.

Cayman’s Glucose Uptake Cell-based Assay Kit provides a convenient tool for studying modulators of cellular glucose uptake. The kit employs 2-NBDG, a fluorescently labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.

2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl) amino]-D-glucose, also known as 2-NBDG, is a fluorescent D-glucose analog. The consequent fluorescent activity is detected by flow cytometry. 2-NBDG is incubated directly with the test sample for uptake, followed by measurement.

2-NBDG is a fluorescent glucose analog and it is used as an indicator for cell viability by monitoring glucose uptake in living cells (Zou et al.). 2-NBDG displays excitation and emission maxima at 465 nm and 540 nm, respectively (Yoshioka et al.).

2-NBDG可以通过葡萄糖转运蛋白进入细胞内，己糖激酶将其磷酸化后滞留在细胞内 [1]。 2-NBDG是一种用于直接监测活细胞和组织对葡萄糖摄取活性的荧光标记葡萄糖类似物 (λex=467nm，λem=542nm)，常用于葡萄糖摄入 (Glucose uptake)实验。 2-NBDG常作为评估细胞葡萄糖摄入的示踪物 [2]；也可以用于肿瘤葡萄糖代谢检测 [3]。 -20°C保存，一年有效。 本产 …