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  1. Preparation of 1 M Dithiothreitol (DTT) Solution

    DTT (DTT, C 4 H 10 O 2 S 2, molecular weight 154.25 g/mole) is also known as Cleland’s reagent. DTT is commonly used for the analysis of proteins as a reducing agent. A high concentration of DTT (50 – 100 mM) can reduce all disulfide bonds (-S-S-) of proteins.

  2. DTT (dithiothreitol) - Thermo Fisher Scientific

    Thermo Scientific DTT (DL-Dithiothreitol; Clelands reagent) is used to stabilize enzymes and other proteins, which possess free sulfhydryl groups. It has been shown to restore activity lost by oxidation of these groups in vitro.

  3. DTT crystalline powder, =97% (Ellman′s reagent), Mr 154.3 - Sigma-Aldrich

    1,4-Dithiothreitol (DTT; 1 mM, 30 min preincubation) produced a small, non-specific potentiation of spasmogenic activity in longitudinal muscle strips of guinea-pig small intestine. A direct comparison of contractile responses elicited by histamine and a range of H1- …

  4. Dithiothreitol - Wikipedia

    Dithiothreitol (DTT) is an organosulfur compound with the formula (CH(OH)CH 2 SH) 2. A colorless compound, it is classified as a dithiol and a diol. DTT is redox reagent also known as Cleland's reagent, after W. Wallace Cleland. [2] The reagent is commonly used in its racemic form. Its name derives from the four-carbon sugar, threose.

  5. NEBuffer Comparison Chart (1X) | NEB

    View the NEBuffer Comparison Chart to compare Buffer components and pH.

  6. When should DTT be added in tissue lysis? - ResearchGate

    DTT is used as reducing agent to prevent the oxydation damage. It is mainly used during the isolation of cytoplasmic proteins. 0,5-1 mM is perfectly safe.

  7. Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (–SH) groups in the reduced state and for quantitative reduction of disulfide (–S–S–)

  8. Dithiothreitol, 1M | Sigma-Aldrich - MilliporeSigma

    Dithiothreitol (DTT), also known as Cleland′s reagent, is a reducing agent containing two thiol functional groups. It serves as a protective regent for thiol groups in protein chemistry studies.

  9. Dithiothreitol (DTT) is a reagent most commonly used to cleave disulfide bonds. The No-Weigh Format of DTT consists of convenient microtube packaging that eliminates the difficulties associated with weighing small quantities of reagent. Puncture foil …

  10. Dithiothreitol (DTT) (1 M) Preparation and Recipe - AAT Bioquest

    Dithiothreitol (DTT) (1 M) preparation guide and recipe. Recipe can be automatically scaled by entering desired final volume. DTT (Dithiothreitol) is a commonly used reducing agent.

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