Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry. First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the

Fluorescein (FITC) derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water-solubility. Fluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications.

FITC is a fluorescein derivative consisting of a fluorescein core and an amine-reactive isothiocyanate moiety. The isothiocyanate group reacts with primary amines forming covalent thiourea bonds that attach fluorescein to the biomolecule.

FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry.

Fluorescein 5-isothiocyanate is the 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Fluorescein isothiocyanate (FITC) is a fluorescent probe that is commonly used in the preparation of conjugates with biological molecules. It has high water solubility, bright fluorescence, and low nonspecific binding with biological tissues.

Mar 17, 2022 · First termed in 1942 by A.H. Coons, FITC is a unique fluorescein molecule having an isothiocyanate reactive group (-N=C=S), which replaced the hydrogen atom located at the base of its structure. In this article, we’ll discuss FITC labeling techniques and provide tips and tricks for maximizing yield.

FITC fluorescein isothiocyanate | C42H22N2O10S2 | CID 131730176 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

Key information: FITC (an amine-reactive version of the parent dye, fluorescein) is a widely-used green fluorescent dye supplied with an isothiocyanate reactive group for the labeling of primary amines.

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm. It is widely used to attach a fluorescent label to proteins via the amine group.