Yui Nishino, 19, uses it every day for her commute to university. She said she was surprised when she first heard that the world’s first 3D-printed station building was going to be built here.

The U4/U6.U5 tri-snRNP is a critical component of the spliceosome complex, and its interaction with target mRNAs is essential for accurate splicing. This interaction ensures that defense-related genes ...

Brr2 is a stable subunit of the U5 snRNP and an unusual member of the Ski2-like subfamily of SF2 helicases, with a tandem array of similarly structured active (N-terminal) and inactive (C-terminal) ...

Following binding of the U4/U6.U5 tri-snRNP, the spliceosome either reverses assembly by discarding tri-snRNP or proceeds to activation by irreversible U4 loss. The major pathway for NTC recruitment ...

In the tri‐snRNP, the U4 and the U6 snRNAs are base‐paired, forming a three‐way junction made up of the U4/U6 stem I, the U4/U6 stem II and the U4 internal stem loop I that is located between stems I ...

Prp3 is an essential U4/U6 di-snRNP-associated protein whose functions and molecular mechanisms in pre-mRNA splicing are presently poorly understood. We show by structural and biochemical analyses ...

U4/U6.U5 tri-snRNP is a 1.5-megadalton pre-assembled spliceosomal complex comprising U5 small nuclear RNA (snRNA), extensively base-paired U4/U6 snRNAs and more than 30 proteins, including the key ...

The U4/U6 subunits PRP3 and Tri-20 are represented by two Arabidopsis homologs, while U4/U6-15.5/SNU13 is likely encoded by three genes as the Tri65 subunit of U4/U6.U5 tri-snRNP. Mutation of U4/U6 ...

The U4/U6-U5 tri–small nuclear ribonucleoprotein (snRNP) is a major, evolutionarily highly conserved spliceosome subunit. Unwinding of its U4/U6 snRNA duplex is a central event of spliceosome ...

Here we focus on the transition from the singular U6 snRNP to the U4/U6 snRNP in the mammalian system. Due to its low abundance in nuclear extract, free U6 snRNP is not very well characterized.