Strengths: The authors employed a battery of next-generation sequencing and crosslinking techniques (e.g., Quick-irCLIP, APA-Seq, and Ribo-Seq) to describe a previously unappreciated binding of eIF3 ...

The authors perform irCLIP of neuronal progenitor cells to profile eIF3-RNA interactions upon short-term neuronal differentiation. The data shows that eIF3 mostly interacts with 3'-UTRs - specifically ...

At the molecular level, IRE1α directly interacts with eIF4G and eIF3, 2 critical components of the translation initiation complex. We demonstrate that IRE1α facilitates the formation of the ...

eIF3, whose subunits are frequently overexpressed in cancer, regulates mRNA translation from initiation to termination, but mRNA‐selective functions of individual subunits remain poorly defined. Using ...

Together, Professor Locker at the University of Surrey and Dr Sweeney at the Pirbright Institute previously uncovered a new mechanism of protein synthesis by dengue virus (DENV) where the cellular ...

An integrative model was derived with full-length Nsp1 on the 40S subunit targeting the dynamic interface between the Nsp1, eIF3, and ribosome complex via the crosslink-derived distance limitations.

Translation initiation in eukaryotes is a multi-step pathway and the most regulated phase of translation. Eukaryotic initiation factor 3 (eIF3) is the largest and most complex of the translation ...

2007; Lee et al., 2015; Smith et al., 2016). The eukaryotic translation initiation factor 3 (eIF3) complex is currently the largest translational initiation complex identified in eukaryotes. To ...

Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI ...